Mycoplasma Pneumoniae （MP）
Izina ryibicuruzwa
HWTS-RT024 Mycoplasma Pneumoniae （MP Kit Kit Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Mycoplasma pneumoniae (MP) ni ubwoko bwa mikorobe ntoya ya prokaryotic, iri hagati ya bagiteri na virusi, ifite imiterere y'utugari ariko nta rukuta rw'akagari. Depite ahanini itera indwara zubuhumekero zabantu, cyane cyane kubana ndetse nabasore. Irashobora gutera umusemburo wa mycoplasma wumuntu, kwandura kwubuhumekero bwabana hamwe numusonga udasanzwe. Kugaragara kwa clinique biratandukanye, ibyinshi muri byo ni inkorora ikabije, umuriro, gukonja, kubabara umutwe, kubabara mu muhogo. Indwara y'ubuhumekero yo hejuru hamwe n'umusonga wa bronchial niwo ukunze kugaragara. Bamwe mu barwayi barashobora kwandura indwara zandurira mu myanya y'ubuhumekero kugeza umusonga ukabije, ibibazo by'ubuhumekero bikabije ndetse n'urupfu.
Umuyoboro
| FAM | Mycoplasma pneumoniae |
| VIC / HEX | Igenzura ryimbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | Sputum 、 Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Amakopi 200 / mL |
| Umwihariko | a act Gukora umusaraba: nta reaktivi yambukiranya hamwe na Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, ibicurane bya Haemophilus, Klebsiella pneumoniae, Staphylococcus aureus, My Staphylococcus aureus Pseudomonas aeruginosa, Acinetobacter baumannii, virusi ya grippe A, virusi ya grippe B, virusi ya Parainfluenza I / II / III / IV, Rhinovirus, Adenovirus, metapneumovirus, virusi yubuhumekero hamwe na acide genomic nucleic. b ability Ubushobozi bwo kurwanya-kwivanga: nta kwivanga kwabayeho mugihe ibintu byabangamiye byageragejwe hamwe nibitekerezo bikurikira: hemoglobine (50mg / L), bilirubin (20mg / dL), mucin (60mg / mL), 10% (v / v) amaraso yumuntu, levofloxacin (10μg / mL), moxifloxacin (0.1g / μg) azithromycine (1mg / mL), Clarithromycine (125μg / mL), erythromycine (0.5g / L), doxycycline (50mg / L), minocycline (0.1g / L). |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe-PCR Sisitemu Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR (Hongshi Medical Technology Co., Ltd.) Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu Yigihe-PCR yo Kumenya (FQD-96A, Ikoranabuhanga rya Hangzhou Bioer) MA-6000 Igihe Cyuzuye Cyumubare Wumukino Wamagare (Suzhou Molarray Co., Ltd.) BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
(1) Icyitegererezo
Gusabwa gukuramo reagent: Macro & Micro-Test Virus ADN / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B, HWTS-3006B) 200µL ya saline isanzwe kumvura yatunganijwe. Gukuramo nyuma bigomba gukorwa ukurikije amabwiriza yo gukoresha. Ingano isabwa yo gukuraho ni 80µL.Bisabwa gukuramo reagent: Gukuramo Acide Nucleic Acide cyangwa Reagent (YDP315-R). Gukuramo bigomba gukorwa byimazeyo ukurikije amabwiriza yo gukoresha. Ingano isabwa yo gukuraho ni 60µL.
(2) Oropharyngeal swab
Gusabwa gukuramo reagent: Macro & Micro-Test Virus ADN / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B, HWTS-3006B) gukuramo bigomba gukorwa ukurikije amabwiriza yo gukoresha. Ingano isabwa gukuramo urugero ni 200µL, naho ingano yo gusabwa ni 80µL.Icyifuzo cyo gukuramo reagent: QIAamp Viral RNA Mini Kit (52904) cyangwa Acide Nucleic Acide cyangwa Isukura Reagent (YDP315-R). Gukuramo bigomba gukorwa byimazeyo ukurikije amabwiriza yo gukoresha. Ingano yo gukuramo urugero rwicyitegererezo ni 140µL, naho ingano yo gusabwa ni 60µL.
