Mycoplasma Pneumoniae (MP)
Izina ry'igicuruzwa
HWTS-RT024 Mycoplasma Pneumoniae（MP） Kit yo Gupima Acide Nucleic (Fluorescence PCR)
Ubumenyi bw'indwara
Mycoplasma pneumoniae (MP) ni ubwoko bw'uturemangingo duto cyane twa prokaryotic, turi hagati ya bagiteri na virusi, dufite imiterere y'uturemangingo ariko nta rukuta rw'uturemangingo. MP ahanini itera indwara z'ubuhumekero ku bantu, cyane cyane ku bana n'urubyiruko. Ishobora gutera indwara ya mycoplasma ku bantu, indwara z'ubuhumekero ku bana ndetse n'indwara ya pneumonia idasanzwe. Ibimenyetso byayo biratandukanye, inyinshi muri zo ni inkorora ikomeye, umuriro, ubukonje, kuribwa umutwe, kubabara mu muhogo. Indwara z'ubuhumekero bwo hejuru n'indwara ya pneumonia yo mu muhogo ni byo bikunze kugaragara cyane. Hari abarwayi bashobora kuva ku ndwara z'ubuhumekero bwo hejuru bakagera ku ndwara ya pneumonia ikomeye, kubabara cyane mu myanya y'ubuhumekero ndetse n'urupfu rushobora kubaho.
Umuyoboro
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Igitambaro cy'umuhogo, agahu k'umunwa |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Kopi 200/mL |
| Ubwihariye | a） Uburyo bwo guhuza: nta buryo bwo guhuza Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacterium tuberculosis, Legionella pneumophila, Pseudomonas aeruginosa, Acinetobacter baumannii, Virusi ya Grippe A, Virusi ya Grippe B, Virusi ya Parainfluenza type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus na human genomic nucleic acid. b）Ubushobozi bwo kurwanya ingaruka mbi: nta ngaruka mbi zibaho iyo ibintu bibangamira bipimwe hakoreshejwe ibi bikurikira: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) amaraso y'umuntu, levofloxacin (10μg/mL), moxifloxacin (0.1g/L), gemifloxacin (80μg/mL), azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Ibikoresho Bikoreshwa | Sisitemu ya PCR ikoreshwa mu gihe nyacyo ya 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.) LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu yo Gupima PCR ya LineGene 9600 Plus mu gihe nyacyo (FQD-96A, ikoranabuhanga rya Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermocycler (Suzhou Molarray Co., Ltd.) Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
(1) Urugero rw'amata
Reagent yo gukuramo isabwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ongeraho 200µL y'umunyu usanzwe ku musemburo watunganyijwe. Nyuma yo gukuramo isabwa gukorwa hakurikijwe amabwiriza yo gukoresha. Ingano yo gukuramo isabwa ni 80µL. Reagent yo gukuramo isabwa: Nucleic Acid Extraction cyangwa Purification Reagent (YDP315-R). Gukuramo isabwa gukorwa hakurikijwe amabwiriza yo gukoresha. Ingano yo gukuramo isabwa ni 60µL.
(2) Igitambaro cyo mu kanwa
Reagent yo gukuramo isabwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha. Ingano y'icyitegererezo isabwa gukurwamo ni 200µL, naho ingano y'icyitegererezo isabwa gukurwamo ni 80µL. Reagent yo gukuramo isabwa gukururwa: QIAamp Viral RNA Mini Kit (52904) cyangwa Nucleic Acid Extraction or Purification Reagent (YDP315-R). Gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha. Ingano y'icyitegererezo isabwa gukurwamo ni 140µL, naho ingano y'icyitegererezo isabwa gukurwamo ni 60µL.
