Virusi ya Monkeypox na aside nucleic yo mu bwoko bwa Typing

Virusi ya Monkeypox na aside nucleic igaragara ku ishusho

Ibisobanuro bigufi:

Iyi mashini ikoreshwa mu gupima aside nucleic nucleic virus ya monkeypox clade I, clade II na monkeypox virus mu mazi y’ibisebe by’abantu, mu isuku y’amaso no mu isuzuma ry’amaraso mu maraso.

Ohereza imeri kuri twe

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Izina ry'igicuruzwa

Kit yo Gupima Virusi ya HWTS-OT202-Monkeypox na Typeing Nucleic Acid Detection Kit (Fluorescence PCR)

Icyemezo

Ubumenyi bw'indwara

Monkeypox (Mpox) ni indwara yandura iterwa na virusi ya Monkeypox (MPXV). MPXV ifite imiterere y'amatafari azengurutse cyangwa imeze nk'uruziga, kandi ni virusi ya ADN ifite imigozi ibiri ifite uburebure bwa 197Kb.^[1]Iyi ndwara yandurira ahanini mu nyamaswa, kandi abantu bashobora kwandurira mu kurumwa n'inyamaswa zanduye cyangwa mu gukora ku maraso, amazi yo mu mubiri no mu bisebe by'inyamaswa zanduye. Iyi virusi ishobora kandi kwandurira mu bantu, cyane cyane binyuze mu bitonyanga by'ubuhumekero mu gihe cyo gukorana imbonankubone igihe kirekire cyangwa mu gukorana n'amazi yo mu mubiri w'umurwayi cyangwa ibintu byanduye.^[2-3]Ubushakashatsi bwagaragaje ko MPXV igizwe n’udukoko tubiri dutandukanye: clade I (yari izwi mbere nka clade yo muri Afurika yo Hagati cyangwa Congo Basin clade) na clade II (yari izwi mbere nka clade yo muri Afurika y’Iburengerazuba). Impinduramatwara ya Congo Basin clade byagaragaye neza ko yandurirana hagati y’abantu kandi ishobora gutera urupfu, mu gihe impinduramatwara ya Africa y’Iburengerazuba itera ibimenyetso byoroheje kandi ifite igipimo gito cyo kwandurirana hagati y’abantu.^[4].

Ibisubizo by'ibizamini by'iyi kit ntibigamije kuba ikimenyetso cyonyine cy'uko abarwayi banduye MPXV, bigomba guhuzwa n'imiterere y'umurwayi ndetse n'andi makuru y'ibizamini bya laboratwari kugira ngo hamenyekane neza ubwandu bw'iyi ndwara no gushyiraho gahunda ikwiye yo kuvura kugira ngo habeho ubuvuzi bwiza kandi butekanye.

Umuyoboro

FAM	MPXV clade II
ROX	aside nucleique rusange ya MPXV
VIC/HEX	MPXV clade I
CY5	igenzura ry'imbere

Ibipimo bya tekiniki

Ububiko	≤-18℃
Igihe cyo kumara	Amezi 12
Ubwoko bw'ingero	amazi yo gushyuha mu maso y'abantu, ifu yo mu kanwa n'amaraso
Ct	≤38 (FAM, VIC/HEX, ROX), ≤35 (IC)
LoD	Kopi 200/mL
Ibikoresho Bikoreshwa	Imashini isuzuma ubwoko bwa I: Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio^®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.) LightCycler^®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu zo Gupima za LineGene 9600 Plus mu gihe nyacyo (FQD-96A, ikoranabuhanga rya Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermocycler (Suzhou Molarray Co., Ltd.) Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo Ubwoko bwa kabiri bw'imashini isuzuma: Eudemon^TMAIO800 (HWTS-EQ007)) yakozwe na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.