Uburasirazuba bwo hagati Ubuhuke Syndrome Coronavirus nucleic acide
Izina ry'ibicuruzwa
HWTS-RT031A-Ububiko bwa Syndrome Ubuhuke bwa Coronavirus Nucleic Gutahura Acide Gutahura (Fluorescent PCR)
Epidemiology
Uburasirazuba bwo hagati Ubuhungiro Coronavirus (Mers-COV), a β-coronarwarus iteraIndwara y'ubuhumekewe mu bantu, yamenyekanye bwa mbere mu murwayi wa nyagasani w'imyaka 60 ya nyakwigendera ku ya 24 cyapfuye. Indwara y'amavukire ya Mers-Cov iriruka ku ndwara y'ibihugu bitangaje cyangwa yoroheje y'indwara z'ubuhumekero kugeza indwara ikomeye y'ubuhumekero ndetse n'urupfu.
Umuyoboro
| Umuryango | Mers virusi rna |
| Vic (hex) | Kugenzura imbere |
Tekinike
| Ububiko | ≤-18 ℃ mu mwijima |
| Imibereho-Ubuzima | Amezi 9 |
| Ubwoko bw'ikigereranyo | Yakusanyije neza nasopharngeal swabs |
| CV | ≤5.0% |
| Ct | ≤38 |
| Lod | Amakopi 1000 / ML |
| Umwihariko | Nta musaraba wambukiranya hamwe na coronasiruki muntu coronavirus sarr-cov hamwe nizindi mwobo usanzwe. |
| Ibikoresho bisabwa: | Gushyira mu bikorwa biosystems 7500 igihe cya sisitemu ya PCR Gushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Quartudio®5 sisitemu nyayo PCR Scan-96p sisitemu nyayo pcr sisitemu Umucyo®480 Igihe Cyigihe cya PCR Linegene 9600 wongeyeho sisitemu yigihe cya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Ihitamo rya 1.
Basabwe gukuramo Reagent: Qiaip viral rna mini kit (52904), acide ya nucleic yatangajwe (YDP315-R) na Tiangen biotech (Beijing) Co., Ltd.
IHitamo 2.
Gusaba gukuramo Reagent: Macro & Micro-Ikizamini Rusange ADN / RNA Kit (HNAT-3017) na Macro & Micro-Ikizamini Automatic Automac Automactor (HWTS-3006C, HWTS-3006B).
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