Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa hamwe n'udukoko twinshi turwanya imiti (KPC, NDM, OXA48 na IMP)
Izina ry'igicuruzwa
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa hamwe n'udukoko twinshi two kurwanya imiti (KPC, NDM, OXA48 na IMP) Multiplex Detection Kit (Fluorescence PCR)
Icyemezo
CE
Ubumenyi bw'indwara
Klebsiella pneumoniae ni indwara ikunze kwibasira abantu benshi ikaba n'imwe muri bagiteri zikomeye zitera indwara zo mu gace k'ubuhumekero. Iyo ubudahangarwa bw'umubiri bugabanutse, bagiteri zinjira mu bihaha ziturutse mu nzira y'ubuhumekero, zigatera ubwandu mu bice byinshi by'umubiri, kandi gukoresha imiti yica udukoko hakiri kare ni byo bivura.[1].
Ahantu hakunze kugaragara ubwandu bwa Acinetobacter baumannii ni ibihaha, ari na byo bitera indwara y’umusonga uterwa n’ibitaro (HAP), cyane cyane umusonga ujyana n’umwuka (VAP). Akenshi ujyana n’izindi ndwara za bagiteri n’ibihumyo, zigaragaza ko indwara nyinshi n’impfu nyinshi.
Pseudomonas aeruginosa ni yo bacilli idatera ubushyuhe ikunze kugaragara cyane mu buvuzi, kandi ni indwara ikomeye itera ubwandu ikwirakwira mu bitaro, ifite imiterere yo koroherwa no kwandura, guhindagurika byoroshye no kudakoresha imiti myinshi.
Umuyoboro
| Izina | PCR-Uruvange rwa 1 | PCR-Mix 2 |
| Umuyoboro wa FAM | Aba | IMP |
| Umuyoboro wa VIC/HEX | Igenzura ry'imbere | KPC |
| Umuyoboro wa CY5 | PA | NDM |
| Umuyoboro wa ROX | KPN | OXA48 |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Amasohoro |
| Ct | ≤36 |
| CV | ≤10.0% |
| LoD | 1000 CFU/mL |
| Ubwihariye | a) Ikizamini cya "cross-reactivity" kigaragaza ko iyi kit idafite "cross reactivity" hamwe n'izindi ndwara zitera indwara zo mu myanya y'ubuhumekero, nka Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus na sputum samples zidafite intego, n'ibindi. b) Ubushobozi bwo kurwanya ingaruka mbi: Hitamo mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, aside clavulanic, na roxithromycin, nibindi kugira ngo upime ingaruka mbi, kandi ibisubizo bigaragaza ko ibintu bibangamira bivuzwe haruguru bitabangamira mu kubona Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa na carbapenem resistance genes KPC, NDM, OXA48 na IMP. |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu yo Gupima PCR ya LineGene 9600 Plus mu gihe nyacyo (FQD-96A, ikoranabuhanga rya Bioer) MA-6000 Real-Time Quantitative Thermocycler (Suzhou Molarray Co., Ltd.) Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Igisubizo cya PCR cyose
