Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa na Genes Resistance Genes (KPC, NDM, OXA48 na IMP) Multiplex
Izina ryibicuruzwa
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii na Pseudomonas Aeruginosa na Gen Resistance Genes (KPC, NDM, OXA48 na IMP) Multiplex Detection Kit (Fluorescence PCR)
Icyemezo
CE
Epidemiologiya
Klebsiella pneumoniae ni indwara isanzwe ivura indwara kandi ni imwe muri bagiteri zikomeye zitera indwara ziterwa na nosocomial. Iyo imbaraga z'umubiri zigabanutse, bagiteri zinjira mu bihaha ziva mu myanya y'ubuhumekero, bigatera kwandura mu bice byinshi by'umubiri, kandi gukoresha hakiri kare antibiyotike ni urufunguzo rwo gukiza[1].
Ahantu hakunze kwibasirwa na Acinetobacter baumannii ni ibihaha, akaba ari yo mpamvu ikomeye itera ibitaro byanduye umusonga (HAP), cyane cyane Ventilator ifitanye isano n'umusonga (VAP). Bikunze guherekezwa nizindi ndwara ziterwa na bagiteri na fungal, hamwe nibiranga umuvuduko mwinshi w’indwara n’impfu nyinshi.
Pseudomonas aeruginosa ni bacili ikunze kutavangwa na ferment-grama-negatif mu bikorwa by’ubuvuzi, kandi ni indwara ikomeye itera indwara zandurira mu bitaro, hamwe n’ibiranga ubukoloni bworoshye, guhinduka byoroshye no kurwanya ibiyobyabwenge byinshi.
Umuyoboro
| Izina | PCR-Kuvanga 1 | PCR-Kuvanga 2 |
| Umuyoboro wa FAM | Aba | IMP |
| Umuyoboro wa VIC / HEX | Igenzura ryimbere | KPC |
| CY5 Umuyoboro | PA | NDM |
| Umuyoboro ROX | KPN | OXA48 |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | Amacandwe |
| Ct | ≤36 |
| CV | ≤10.0% |
| LoD | 1000 CFU / mL |
| Umwihariko | a) Ikizamini cyambukiranya imipaka cyerekana ko iki gikoresho kitagira umusemburo hamwe nizindi ndwara zandurira mu myanya y'ubuhumekero, nka Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus grippe, Acinetobacter jelly, Acinetobacter jelly, Acinetobacter jelly, Acinetobacter jelly, Acinetobacter jelly, Acinetobacter jelly, Acinetobacter jelly, Acinetobacter jelly, Acinetobacter hemolytica Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Adenovirus yubuhumekero, Enterococcus na sputum ntangarugero, nibindi. b) Ubushobozi bwo kurwanya-kwivanga: Hitamo mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, acide clavulanic, na roxithromycine, nibindi kugirango ibizamini bivangavanze, hamwe nibisubizo byerekana ko kwivanga kwa interineti kwerekanwa kwivanga, Acinetobacter baumannii, Pseudomonas aeruginosa na karbapenem irwanya genes KPC, NDM, OXA48 na IMP. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu nyayo-PCR Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu Yigihe-PCR yo Kumenya (FQD-96A, Ikoranabuhanga rya Bioer) MA-6000 Igihe Cyuzuye Cyumubare Wumukino Wamagare (Suzhou Molarray Co., Ltd.) BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Igisubizo cya PCR
