Ibicurane virusi kwisi yose / h1 / h3
Izina ry'ibicuruzwa
HWTS-RT012 Grippeza A virusi kwisi yose / H1 / H3 Nucleic acide ibishushanyo byinshi byatahura (fluorescence pcr)
Epidemiology
Virusi y'ibihe bibi ni amoko ahiga ya ORTHOMYXOVRIDAE. Ni indwara ya iterabwoba ubuzima bwa muntu. Irashobora kwanduza uwakiriye cyane. Icyorezo cyibihe kigira ingaruka kubantu bagera kuri miliyoni 600 kwisi yose kandi bigatera impfapfa 250.000 ~ 500.000, muri zo ibicurane bizagira uruhare runini rwo kwandura no gupfa. Ibicurane Ir virusi ni rna imwe idahwitse. Ukurikije ubuso bwayo Hemaggletinin (ha) na neuragesease (NA), ha irashobora kugabanywamo 16 subtypes, na zigabanyijemo subtypes 9. Among influenza A viruses, the subtypes of influenza viruses that can directly infect humans are: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 and H10N8. Muri bo, H1 na H3 subtypes ni pathogenic, kandi bakwiriye kwitabwaho cyane.
Umuyoboro
| Umuryango | Ibicurane Ubwoko bwa virusi rusange |
| Vic / Hex | Ibicurane Acide ya virusi ya H1 |
| Rox | Ibicurane Acide ya virusi ya H3 |
| Cy5 | kugenzura imbere |
Tekinike
| Ububiko | ≤-18 ℃ |
| Imibereho-Ubuzima | Amezi 9 |
| Ubwoko bw'ikigereranyo | Nasopharyngeal Swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| Lod | Kopi 500 / μl |
| Umwihariko | Nta musaruro wo kurengana n'izindi ngero z'ubuhumekero nka Grippe A1 / A2 / B1 / B2, Virusi yubuhumekene A / B, Coronavirus 229E / NL63, Rhinovirus A / B / COLAVIRUS Trachomatis, Adenovirus, nibindi ADN ya Genomic. |
| Ibikoresho bisabwa | Gukoresha Biosystems 7500 Igihe cya PCR Gushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Quartudio®5 sisitemu nyayo PCR Slan-96p Sisitemu NYAKURI PCR (Tekinoroji yubuvuzi Cology Co, Ltd.) Umucyo®480 Igihe Cyigihe cya PCR Umurongo 9600 wongeyeho sisitemu yigihe cya PCR (FQD-96a, Hangzhou tekinoroji yibinyabuzima) Ma-6000 nyayo-nyayo Umwanya wa Cyrmal Cycler (Suzhou Morlary Co, Ltd.) Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Gukuramo aciccction cyangwa kweza reagent (YDP315-R) na Tiangen biotech (Beijing) Co, Ltd. Gukuramo bigomba gukorwa neza ukurikije amabwiriza yo gukoresha. Igitabo cyitegererezo cyakuweho ni 140μl, kandi umubumbe wabisabwa ni 60μl.
IHitamo 2.
Macro & Micro-Ikizamini cya virusi ya VNA / RNA (HWTS-3004-32, HWTS-3004-4-96) na macro & micro-Ikizamini Automatic Automatic Automatic, HWTS-3006B). Gukuramo bigomba gukorwa neza ukurikije amabwiriza yo gukoresha. Igitabo cyitegererezo cyakuweho ni 200μl, hamwe nubunini bwabigenewe ni 80μl.
