Ibicurane virusi rusange / H1 / H3
Izina ryibicuruzwa
HWTS-RT012 Ibicurane Virusi Yose / H1 / H3 Acide Nucleic Acide Multiplex Detection Kit (Fluorescence PCR)
Epidemiologiya
Virusi y'ibicurane ni ubwoko bwerekana Orthomyxoviridae. Ni indwara yangiza cyane ubuzima bwabantu. Irashobora kwanduza uwakiriye cyane. Icyorezo cyibihe cyibasira abantu bagera kuri miriyoni 600 kwisi yose kandi gitera impfu 250.000 ~ 500.000, muri zo virusi ya grippe A niyo nyirabayazana yo kwandura no gupfa. Ibicurane A ni virusi imwe ya RNA itemewe. Ukurikije ubuso bwacyo hemagglutinin (HA) na neuraminidase (NA), HA irashobora kugabanywamo amoko 16, NA Igabanyijemo 9. Muri virusi ya grippe A, ubwoko bwa virusi yibicurane bushobora kwanduza abantu ni: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 na H10N8. Muri byo, ubwoko bwa H1 na H3 butera indwara cyane, kandi bukwiye kwitabwaho.
Umuyoboro
| FAM | ibicurane Ubwoko bwa virusi nucleic aside |
| VIC / HEX | ibicurane A H1 ubwoko bwa virusi nucleic aside |
| ROX | ibicurane A H3 ubwoko bwa virusi nucleic aside |
| CY5 | kugenzura imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 9 |
| Ubwoko bw'icyitegererezo | nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Amakopi 500 / μL |
| Umwihariko | Nta reaction-reaction hamwe nizindi ngero zubuhumekero nka Grippe A, Grippe B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, virusi yubuhumekero, Parainfluenza 1, 2, 3, virusi ya Coxsackie, Echo virusi, Metapneum2 virusi A / B, Coronavirus 229E / NL63 / HKU1 / OC43, Rhinovirus A / B / C, virusi ya Boca 1/2/3/4, Chlamydia trachomatis, adenovirus, nibindi na ADN ya ADN. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe-PCR Sisitemu Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR SLAN-96P Sisitemu nyayo-PCR (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu Yigihe-PCR yo Kumenya (FQD-96A, Ikoranabuhanga rya Hangzhou Bioer) MA-6000 Igihe Cyuzuye Cyumubare Wumukino Wamagare (Suzhou Molarray Co., Ltd.) BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Gukuramo Acide Nucleic cyangwa Kweza Reagent (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd. Gukuramo bigomba gukorwa cyane ukurikije amabwiriza yo gukoresha. Ingano yicyitegererezo yakuweho ni 140μL, naho ingano yo gusabwa ni 60μL.
Icya 2.
Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acide (HWTS-3006C, HWTS-3006B). Gukuramo bigomba gukorwa cyane ukurikije amabwiriza yo gukoresha. Ingano yicyitegererezo yakuweho ni 200μL, naho icyifuzo cyo gukuraho ni 80μL.
