Ibicurane virusi rusange / H1 / H3
Izina RY'IGICURUZWA
HWTS-RT012 Ibicurane Virusi Yose / H1 / H3 Acide Nucleic Acide Multiplex Detection Kit (Fluorescence PCR)
Epidemiologiya
Mycoplasma pneumoniae (MP) ni mikorobe ntoya ya prokaryotic ifite imiterere ya selile kandi nta rukuta rw'akagari hagati ya bagiteri na virusi.Depite ahanini itera indwara zifata imyanya y'ubuhumekero ku bantu, cyane cyane ku bana no mu rubyiruko.Umudepite ashobora gutera Mycoplasma hominis umusonga, indwara zifata imyanya y'ubuhumekero ku bana ndetse n'umusonga udasanzwe.Ibimenyetso bya clinique biratandukanye, cyane cyane inkorora ikabije, umuriro, gukonja, kubabara umutwe, kubabara mu muhogo, kwandura mu myanya y'ubuhumekero no kuri bronchopneumoniya ni byo bikunze kugaragara.Bamwe mu barwayi barashobora kurwara umusonga bikabije bivuye mu myanya y'ubuhumekero yo hejuru, kandi hashobora kubaho ibibazo by'ubuhumekero cyangwa urupfu.Umudepite ni umwe mu ndwara zitera kandi zikomeye mu musonga wanduye abaturage (CAP), zingana na 10% -30% bya CAP, kandi umubare ushobora kwiyongera inshuro 3-5 mugihe umudepite yiganje.Mu myaka yashize, umubare w’abadepite muri CAP wanduye wiyongereye buhoro buhoro.Indwara ya Mycoplasma pneumoniae yiyongereye, kandi kubera ivuriro ryayo ridafite akamaro, biroroshye kwitiranya ibicurane bya bagiteri na virusi.Kubwibyo, gutahura laboratoire hakiri kare bifite akamaro kanini mugupima no kuvura.
Umuyoboro
| FAM | ibicurane Ubwoko bwa virusi nucleic aside |
| VIC / HEX | ibicurane A H1 ubwoko bwa virusi nucleic aside |
| ROX | ibicurane A H3 ubwoko bwa virusi nucleic aside |
| CY5 | kugenzura imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 9 |
| Ubwoko bw'icyitegererezo | nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Amakopi 500 / μL |
| Umwihariko | Nta reaction-reaction hamwe nizindi ngero zubuhumekero nka Grippe A, Grippe B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, virusi yubuhumekero, Parainfluenza 1, 2, 3, virusi ya Coxsackie, Echo virusi, Metapneumovir B1 / B2, virusi yubuhumekero A / B, Coronavirus 229E / NL63 / HKU1 / OC43, Rhinovirus A / B / C, virusi ya Boca 1/2/3/4, Chlamydia trachomatis, adenovirus, nibindi na ADN ya ADN. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe-PCR Sisitemu Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR SLAN-96P Sisitemu nyayo-PCR Sisitemu (Hongshi Medical Technology Co., Ltd.) UmucyoCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu Yigihe-PCR yo Kumenya (FQD-96A, Ikoranabuhanga rya Hangzhou Bioer) MA-6000 Igihe Cyuzuye Cyumubare Wumukino Wamagare (Suzhou Molarray Co., Ltd.) BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Gukuramo Acide Nucleic cyangwa Kweza Reagent (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd.Gukuramo bigomba gukorwa cyane ukurikije amabwiriza yo gukoresha.Ingano yicyitegererezo yakuweho ni 140μL, naho ingano yo gusabwa ni 60μL.
Icya 2.
Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acide (HWTS-3006C, HWTS-3006B).Gukuramo bigomba gukorwa cyane ukurikije amabwiriza yo gukoresha.Ingano yicyitegererezo yakuweho ni 200μL, naho icyifuzo cyo gukuraho ni 80μL.
