Virusi ya Grippe A Universal/H1/H3
Izina ry'igicuruzwa
Kit yo Gupima A Virus ya HWTS-RT012 Influenza A Universal/H1/H3 Nucleic Acid Multiplex (Fluorescence PCR)
Ubumenyi bw'indwara
Virusi ya Grippe A ni ubwoko bw’indwara ya Orthomyxoviridae. Ni indwara itera indwara ibangamira ubuzima bw’abantu cyane. Ishobora kwanduza umuntu cyane. Icyorezo cy’ibihe cy’icyorezo cyibasira abantu bagera kuri miliyoni 600 ku isi yose, kikaba gitera impfu 250.000 kugeza 500.000, muri zo virusi ya Grippe A ikaba ari yo mpamvu nyamukuru y’ubwandu n’urupfu. Virusi ya Grippe A ni RNA ifite umugozi umwe. Dukurikije hemagglutinin yayo yo hejuru (HA) na neuraminidase (NA), HA ishobora kugabanywamo ubwoko 16, NA Igabanyijwemo ubwoko 9. Muri virusi za Grippe A, ubwoko bwa virusi za Grippe A zishobora kwanduza abantu ni: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 na H10N8. Muri zo, ubwoko bwa H1 na H3 buratera indwara cyane, kandi bukwiye kwitabwaho cyane.
Umuyoboro
| FAM | aside nucleic ya virusi yo mu bwoko bwa grippe |
| VIC/HEX | aside nucleic ya virusi yo mu bwoko bwa grippe A H1 |
| ROX | aside nucleic ya virusi yo mu bwoko bwa grippe A H3 |
| CY5 | igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 9 |
| Ubwoko bw'ingero | agapfukamunwa ko mu mazuru |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Kopi 500/μL |
| Ubwihariye | Nta ndwara iterwa n’izindi ndwara z’ubuhumekero nka Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, nibindi na ADN y’uturemangingo tw’umubiri w’umuntu. |
| Ibikoresho Bikoreshwa | Sisitemu ya PCR ikoreshwa mu gihe nyacyo ya 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 Sisitemu za PCR za QuantStudio®5 mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.) Sisitemu ya LightCycler®480 Real-Time PCR Sisitemu yo Gupima PCR ya LineGene 9600 Plus mu gihe nyacyo (FQD-96A, ikoranabuhanga rya Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermocycler (Suzhou Molarray Co., Ltd.) Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Gukuramo aside ya Nucleic cyangwa Reagent yo Gusukura (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd. Gukuramo bigomba gukorwa neza hakurikijwe amabwiriza yo gukoresha. Ingano y'icyitegererezo cyakuwemo ni 140μL, naho ingano ya elution isabwa ni 60μL.
Amahitamo ya 2.
Kit ya Macro & Micro-Test Viral ADN/RNA (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Gukuramo bigomba gukorwa neza hakurikijwe amabwiriza yo gukoresha. Ingano y'icyitegererezo cyakuwemo ni 200μL, naho ingano ya elution isabwa ni 80μL.
