Umuntu PML-RARA Fusion Gene Mutation
Izina ryibicuruzwa
HWTS-TM017AUmuntu PML-RARA Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Epidemiologiya
Acute promyelocytic leukemia (APL) ni ubwoko bwihariye bwa acute myeloid leukemia (AML). Abagera kuri 95% by'abarwayi ba APL baherekejwe n'impinduka idasanzwe ya cytogenetike, ari yo t (15; 17) (q22; q21), ituma gene ya PML kuri chromosome 15 hamwe na reseptor acide retinoic α gene (RARA) kuri chromosome 17 yahujwe no gukora gene ya PML-RARA. Bitewe no gutandukana kwa gene ya PML, gene ya PML-RARA ishobora kugabanywa mubwoko burebure (ubwoko bwa L), ubwoko bugufi (S ubwoko) nubwoko butandukanye (V ubwoko), bingana na 55%, 40% na 5%.
Umuyoboro
| FAM | PML-RARA fusion gene |
| ROX | Igenzura ryimbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ Mu mwijima |
| Ubuzima bwa Shelf | Amezi 9 |
| Ubwoko bw'icyitegererezo | amagufwa |
| CV | <5.0 ％ |
| LoD | Amakopi 1000 / mL. |
| Umwihariko | Nta re-reactivité hamwe nizindi genes zo guhuza BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, na TEL-AML1 gen. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR Sisitemu (Hongshi Medical Technology Co., Ltd.) Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu Yigihe-PCR yo Kumenya (FQD-96A, Ikoranabuhanga rya Hangzhou Bioer) MA-6000 Igihe Cyuzuye Cyumubyigano Wumuriro (Suzhou Molarray Co., Ltd.) BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu nyayo-PCR |
Urujya n'uruza rw'akazi
Gusabwa gukuramo reagent: RNAprep Amaraso Yuzuye Igikoresho cyo gukuramo RNA (DP433). Gukuramo bigomba gukorwa ukurikije IFU.
