Umuntu cytomegalovirus (hcmv) acide nucleic
Izina ry'ibicuruzwa
Hwts-ur008A-Umuntu Cytomegalovirus (HCMV) Acide ya Acide ya Acicction Kit (Fluorescence PCR)
Epidemiology
Umuntu cytomegalovirus (HCMV) ni umunyamuryango wa genome nini mumuryango wa virusi ya herpes kandi ushobora gushiramo poroteine zirenze 200. HCMV igarukira gato muburyo bwakiriwe kubantu, kandi nta cyitegererezo cyinyamanswa cyanduye. HCMV ifite umuvuduko gahoro kandi muremure kugirango ukore umubiri wintwari, kandi utegereho umusaruro wa perninuclear na cytoplasic imibiri hamwe na selile ibyimba (selile nini), niyo mpamvu. Dukurikije urwibutso rwa genogene na phenotype, hcmv irashobora kugabanwa muburyo butandukanye, muri bo harimo itandukaniro rya Antigenic, ariko, nta busobanuro bwa kuvura.
Kwandura hcmv ni indwara ya sisitemu, ikubiyemo ibimenyetso byinshi, ifite ibimenyetso byinshi kandi bitandukanye cyane, kandi birashobora gutuma abarwayi bake batezimbere imirongo myinshi barimo retinitis, kandi irashobora guceceka, kandi ikigoshe, monocytose, na trombocytopenic Pupura. Indwara ya HCMV irasanzwe kandi igaragara ikwirakwira kwisi yose. Izirimba cyane mu baturage, hamwe n'ibipimo by'Icyubahiro bya 45-50% n'ibirenze 90% mu bihugu byateye imbere kandi bikiri mu nzira y'amajyambere. HCMV irashobora gushiramo gusinzira mumubiri igihe kirekire. Ubudahangarwa bw'umubiri bumaze gucika intege, virusi izakorwa kugira ngo itera indwara, cyane cyane indwara zinjira mu barwayi ba Leukemia n'abarwayi bahinduwe, kandi ishobora gutera urufatiro rwabarwayi kandi rushobora kubangamira ubuzima bw'abarwayi mu manza zikomeye. Usibye kubyara, gukuramo inda no gutanga imburagihe hashobora kandi gutera imikorere ya selforming, kwandura HCMV birashobora kugira ingaruka ku buvuzi bwo kubyara no kubyara no kubyara.
Umuyoboro
Umuryango | Hcmv dna |
Vic (hex) | Kugenzura imbere |
Tekinike
Ububiko | Amazi: ≤-18 ℃ mu mwijima |
Imibereho-Ubuzima | Amezi 12 |
Ubwoko bw'ikigereranyo | Icyitegererezo cya Serum, icyitegererezo cya Plasma |
Ct | ≤38 |
CV | ≤5.0% |
Lod | Amakopi / reaction |
Umwihariko | Nta musaraba wambukiranya na Hepatite B virusi, virusi ya hepatite c, virusi ya Papilloma, Herpes Synopx virusi 1, Herpes ShoftX virusi ya 1, Herpes Synopx virusi ya 2, Erpes Symbolx virusi ya 2, nibindi |
Ibikoresho bisabwa: | Irashobora guhuza ibikoresho nyamukuru bya PCR ku isoko. Gushyira mu bikorwa biosystems 7500 igihe cya sisitemu ya PCR Gushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Quartudio®5 sisitemu nyayo PCR Scan-96p sisitemu nyayo pcr sisitemu Umucyo®480 Igihe Cyigihe cya PCR Linegene 9600 wongeyeho sisitemu yigihe cya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Hwts-3017-50, hwts-3017-32, HWTS-3017-48, HWTS-GUKORESHA ACCO & PORT-3006C, HWTS-3006B)) na Jiagsu Macro & Micro-Ikizamini Med-Tech Co., Ltd. Gukuramo bigomba gukururwa ukurikije amabwiriza. Gukuramo icyitegererezo ni 200μl, hamwe nububiko bwabigenewe ni 80μl.
Gusaba gukuramo Reagent: Qiaimp dna Mini Kit (51304), acide ya nucleic cyangwa isukura reagent (YDP315) na Tiangen biotech (Beijing) Co. bigomba gukurwa ukurikije amabwiriza yo gukuramo, kandi umubumbe wasabye ni 200 μl hamwe nububiko bwabigenewe ni 100 μl.