Umuntu Cytomegalovirus (HCMV) Acide Nucleic
Izina RY'IGICURUZWA
HWTS-UR008A-Cytomegalovirus yumuntu (HCMV) ibikoresho byo gutahura aside nucleic (Fluorescence PCR)
Epidemiologiya
Umuntu cytomegalovirus (HCMV) ni umunyamuryango ufite genome nini mu muryango wa virusi ya herpes kandi irashobora gushiramo poroteyine zirenga 200.HCMV irabujijwe cyane mubakira abantu, kandi haracyariho inyamaswa zanduye.HCMV ifite uburyo bwo kwigana buhoro kandi burebure bwo gukora umubiri winjizamo ingufu za kirimbuzi, kandi bigatera kubyara umubiri wa perinuclear na cytoplasmeque hamwe no kubyimba ingirabuzimafatizo (selile nini), bityo izina.Ukurikije itandukanyirizo rya genome na fenotype, HCMV irashobora kugabanywamo amoko atandukanye, muri yo hakaba harimo itandukaniro rya antigenic, ariko, nta kamaro k’ubuvuzi.
Indwara ya HCMV ni indwara yanduye, ifata ingingo nyinshi, ifite ibimenyetso bigoye kandi bitandukanye, ahanini iraceceka, kandi irashobora gutuma abarwayi bake barwara ibisebe byinshi birimo retinite, hepatite, umusonga, encephalite, colitis, monocytose, na trombocytopenic purpura.Indwara ya HCMV irasanzwe cyane kandi bigaragara ko ikwira isi yose.Yiganje cyane mu baturage, aho umubare w'ababana na 45-50% urenga 90% mu bihugu byateye imbere kandi bikiri mu nzira y'amajyambere.HCMV irashobora kuryama mumubiri igihe kirekire.Ubudahangarwa bw'umubiri nibumara gucika intege, virusi izakorwa kugirango itere indwara, cyane cyane indwara zisubirwamo ku barwayi ba leukemia ndetse n’abarwayi batewe, kandi zishobora gutera indwara ya nérosose yatewe kandi ikabangamira ubuzima bw’abarwayi mu bihe bikomeye.Usibye kubyara, gukuramo inda no kubyara imburagihe binyuze mu kwandura intrauterine, cytomegalovirus irashobora kandi gutera indwara zivuka, bityo indwara ya HCMV ikaba ishobora kugira ingaruka ku buvuzi butwite na nyuma yo kubyara ndetse n'ubwiza bw'abaturage.
Umuyoboro
FAM | ADN ya ADN |
VIC (HEX) | Igenzura ryimbere |
Ibipimo bya tekiniki
Ububiko | Amazi: ≤-18 ℃ Mu mwijima |
Ubuzima bwa Shelf | Amezi 12 |
Ubwoko bw'icyitegererezo | Icyitegererezo cya Serumu, Icyitegererezo cya Plasma |
Ct | ≤38 |
CV | ≤5.0% |
LoD | Amakopi / reaction |
Umwihariko | Nta reaktivi ihura na virusi ya hepatite B, virusi ya hepatite C, virusi ya papilloma yumuntu, virusi ya herpes simplex ubwoko bwa 1, virusi ya herpes simplex ubwoko bwa 2, ingero zisanzwe za serumu zabantu, nibindi. |
Ibikoresho bikoreshwa: | Irashobora guhuza ibikoresho nyamukuru bya fluorescent PCR kumasoko. Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR SLAN-96P Sisitemu nyayo-PCR UmucyoCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co, Ltd .. Gukuramo bigomba gukururwa ukurikije amabwiriza.Ingano yo gukuramo urugero ni 200μL, naho icyifuzo cyo gukuraho ni 80μL.
Gusabwa gukuramo reagent: QIAamp ADN Mini Kit (51304), Gukuramo Acide Nucleic Acide cyangwa Isukura Reagent (YDP315) na Tiangen Biotech (Beijing) Co., Ltd.bigomba gukururwa ukurikije amabwiriza yo gukuramo, kandi ingano isabwa gukuramo ni 200 μL naho icyifuzo cyo gukuraho ni 100 μL.