Herpes Simplex Virus Ubwoko bwa 2 Nucleic Acide
izina RY'IGICURUZWA
HWTS-UR007A-Herpes Simplex Virus Ubwoko bwa 2 Nucleic Acide Detection Kit (Fluorescence PCR)
Gukoresha
Iki gikoresho gikoreshwa mugushakisha ubuziranenge bwa herpes simplex virusi yo mu bwoko bwa 2 nucleic acide muri urethral swab yabagabo na servical swab sample.
Epidemiologiya
Herpes Simplex Virus Ubwoko bwa 2 (HSV2) ni virusi yumuzingi ikomatanyirizwa hamwe na tegument, capsid, intangiriro, hamwe n ibahasha, kandi irimo ADN ifite imirongo ibiri.Virusi ya Herpes irashobora kwinjira mumubiri binyuze muburyo butaziguye cyangwa guhuza ibitsina nuruhu nuduce twinshi, kandi bigabanijwemo ibanze kandi bigaruka.Indwara yimyororokere iterwa ahanini na HSV2, abarwayi b'igitsina gabo bagaragara nk'ibisebe by'imboro, naho abarwayi b'igitsina gore bagaragara nk'ibisebe by'inkondo y'umura, inkondo y'umura, n'inda ibyara.Indwara ya mbere ya virusi ya herpes ni indwara zanduye, usibye herpes nkeya zaho zifite ururenda cyangwa uruhu, inyinshi muri zo zikaba nta bimenyetso bigaragara by’amavuriro.Indwara ya herpes yanduye ifite ibiranga virusi ubuzima bwose itwara kandi igaruka byoroshye, kandi abarwayi nabatwara ni isoko yandura indwara.Mu Bushinwa, igipimo cyiza cya HSV2 ni 10.80% kugeza 23.56%.Icyiciro cyo kwandura HSV2 kirashobora kugabanywa kwandura kwambere no kwandura inshuro nyinshi, kandi hafi 60% byabarwayi banduye HSV2 barongera.
Epidemiologiya
FAM: Herpes Simplex Virus Ubwoko bwa 2 (HSV2) ·
VIC (HEX): Igenzura ryimbere
Gushiraho PCR Kwiyongera
Intambwe | Amagare | Ubushyuhe | Igihe | KusanyaFluorescentSincyangwa Oya |
1 | 1 Ukuzenguruka | 50 ℃ | 5min | No |
2 | 1 Ukuzenguruka | 95 ℃ | 10min | No |
3 | 40 Amagare | 95 ℃ | 15segonda | No |
4 | 58 ℃ | 31segonda | Yego |
Ibipimo bya tekiniki
Ububiko | |
Amazi | ≤-18 ℃ Mu mwijima |
Ubuzima bwa Shelf | Amezi 12 |
Ubwoko bw'icyitegererezo | Abagore b'inkondo y'umura, Abagabo urethral swab |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50Kopi / reaction |
Umwihariko | Nta reaction-reaction hamwe nizindi ndwara ziterwa na STD, nka Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium nibindi. |
Ibikoresho bikoreshwa | Irashobora guhuza ibikoresho nyamukuru bya fluorescent PCR kumasoko. Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR SLAN-96P Sisitemu nyayo-PCR UmucyoCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu nyayo-PCR. |