Herpes ShoftX Virusi Ubwoko 2 nucleic acide
Izina ry'ibicuruzwa
Hwts-ur007A-Herpes ShoftX virusi ya 2 nucleic Gutahura acide ya Acide Gutahura (Fluorescence PCR)
Gukoresha
Ibi bikoresho bikoreshwa mu kumenya ibyiza bya Herpes Synopx virusi Ubwoko 2 nucleic acide muri Urethral Swab nigitsina gore Swings swing sntergles.
Epidemiology
Herpes Shoftx virusi ya 2 (HSV2) ni virusi izenguruka hanwa na tegument, capsid, intangiriro, ibahasha, kandi irimo umurongo wikubye kabiri. Virusi ya Herpes irashobora kwinjira mumubiri binyuze mubusambanyi cyangwa guhuza ibitsina hamwe nimpu na mucous memshyanes, kandi bigagabanijwemo amabambere kandi bigaruka. Ubwandu bwibihe byimyororokere biterwa ahanini na HSV2, abarwayi bagaragarira mu bisebe bingana, kandi abarwayi b'abagore bagaragarizwa nk'igituba, valvari, n'uduteye. Indwara zambere za virusi yimyanya ndangagitsina ninzitizi zidasanzwe, usibye forpes nkeya hamwe na mucous cyangwa uruhu, ibyinshi bidafite ibimenyetso bifatika. Kwandura Herpes bifite ibintu biranga virusi ubuzima bwabo bwose bitwaye kandi byoroshye, kandi abarwayi na barwayi bombi ni isoko yo kwandura indwara. Mu Bushinwa, igipimo cyiza cya HSV2 gifite imyaka 10.80% kugeza 23.56%. Intambwe yanduye HSV2 irashobora kugabanywamo kwandura abanza kandi yisubirwamo, kandi hafi 60% ya barwayi ba HSV2 banduye.
Epidemiology
Fam: Herpes ShoftX Virusi Ubwoko bwa 2 (HSV2) ·
Vic (Hex): Kugenzura imbere
Imiterere ya PCR igenamiterere
Intambwe | Inzinguzingo | Ubushyuhe | Igihe | KusanyaFLuorescentSIgnalscyangwa ntabwo |
1 | 1 cycle | 50 ℃ | 5mins | No |
2 | 1 cycle | 95 ℃ | 10min | No |
3 | Inzongu 40 | 95 ℃ | 15se | No |
4 | 58 ℃ | 31Secs | Yego |
Tekinike
Ububiko | |
Amazi | ≤-18 ℃ mu mwijima |
Imibereho-Ubuzima | Amezi 12 |
Ubwoko bw'ikigereranyo | Umugore Swircal Swab, Urethral Swab |
Ct | ≤38 |
CV | ≤5.0% |
Lod | 50copies / reaction |
Umwihariko | Nta musaraba ugendana nandi page ya STD, nka Treponema Pallidum, Ureaplasma Ureliyani, Ureaplasma Ureliyani, Mycoplasma Hominis, Mycoplasma diyanium na nibindi. |
Ibikoresho bisabwa | Irashobora guhuza ibikoresho nyamukuru bya PCR ku isoko. Gushyira mu bikorwa biosystems 7500 igihe cya sisitemu ya PCR Gushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Quartudio®5 sisitemu nyayo PCR Scan-96p sisitemu nyayo pcr sisitemu Umucyo®480 Igihe Cyigihe cya PCR Linegene 9600 wongeyeho sisitemu yigihe cya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad cfx opus 96 sisitemu nyayo pcr sisitemu. |