Herpes Simplex Virus Ubwoko 1/2 , (HSV1 / 2) Acide Nucleic
Izina RY'IGICURUZWA
HWTS-UR018A-Herpes simplex virusi ubwoko bwa 1/2, (HSV1 / 2) ibikoresho byo gutahura aside nucleic (Fluorescence PCR)
Epidemiologiya
Indwara zandurira mu mibonano mpuzabitsina (STD) ziracyari imwe mu mbogamizi zikomeye ku mutekano w’ubuzima rusange ku isi.Indwara nkizo zirashobora gutera ubugumba, kubyara imburagihe, kubyimba nibibazo bikomeye.Hariho ubwoko bwinshi bwa virusi itera indwara zandurira mu mibonano mpuzabitsina, harimo bagiteri, virusi, chlamydia, mycoplasma na spirochette, muri zo hakaba harimo Neisseria gonorrhoeae, Mycoplasma genitalium, Chlamydia trachomatis, HSV1, HSV2, Mycoplasma hominis, na Ureaplasma urealyticum.
Indwara ya herpes ni indwara ikwirakwizwa mu mibonano mpuzabitsina iterwa na HSV2, yanduye cyane.Mu myaka ya vuba aha, ubwiyongere bw'imyanya ndangagitsina bwiyongereye ku buryo bugaragara, kandi kubera kwiyongera kw'imyitwarire y’imibonano mpuzabitsina ishobora guteza akaga, igipimo cyo gutahura HSV1 mu myanya ndangagitsina cyiyongereye kandi bivugwa ko kiri hejuru ya 20% -30%.Ubwandu bwa mbere bwanduye virusi ya herpes ahanini buceceka nta bimenyetso bigaragara byamavuriro usibye herpes zaho muri mucosa cyangwa uruhu rwabarwayi bake.Kubera ko herpes imyanya ndangagitsina irangwa no kumara virusi ubuzima bwawe bwose no kugaragara ko byongeye kubaho, ni ngombwa gusuzuma virusi byihuse kandi bikabuza kwanduza.
Umuyoboro
FAM | HSV1 |
CY5 | HSV2 |
VIC (HEX) | Igenzura ryimbere |
Ibipimo bya tekiniki
Ububiko | Amazi: ≤-18 ℃ Mu mwijima |
Ubuzima bwa Shelf | Amezi 12 |
Ubwoko bw'icyitegererezo | ururenda rwa urethral, ururenda rwinkondo y'umura |
Ct | ≤38 |
CV | ≤5.0% |
LoD | Amakopi / reaction |
Umwihariko | Nta reaction-reaction hamwe nizindi ndwara ziterwa na STD nka Treponema pallidum, Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, na Ureaplasma urealyticum. |
Ibikoresho bikoreshwa | Irashobora guhuza ibikoresho nyamukuru bya fluorescent PCR kumasoko. Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe-PCR Sisitemu Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Basabwe gukuramo reagent: Macro & Micro-Ikigereranyo Cyitegererezo cyo Gusohora Reagent (HWTS-3005-8).Gukuramo bigomba gukorwa neza ukurikije amabwiriza.
Gusabwa gukuramo reagent: Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa na Macro & Micro-Ikizamini Gukuramo Acide Nucleic Acide (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Gukuramo bigomba gukorwa cyane hakurikijwe amabwiriza.Ingano isabwa yo gukuraho igomba kuba 80 μL.
Gusabwa gukuramo reagent: Gukuramo Acide Nucleic Acide cyangwa Isukura Reagent (YDP315) na Tiangen Biotech (Beijing) Co, Ltd.Gukuramo bigomba gukorwa neza ukurikije amabwiriza.Ingano isabwa yo gukuraho igomba kuba 80 μL.