Hepatite e virusi
Izina ry'ibicuruzwa
Hwts-hp006 hepatite e virusi nucleic acide gutahura ibikoresho (fluorescence pcr)
Epidemiology
Hepatite E virusi (Hev) ni virusi ya RNA itera ibibazo byubuzima bwisi. Ifite intera ndende kandi ifite umutungo wo kwambuka interspetions. Nimwe mubintu byingenzi bya zoonotic byingenzi kandi bikagirira nabi abantu ninyamaswa. Hev yanduzwa cyane cyane binyuze muri kwanduza imyenda-ocal-ocal, kandi irashobora no kwandura ihagaritse binyuze mu nsoro cyangwa amaraso. Muri bo, mu nzira yanduye, Hev-wanduye amazi n'ibiryo bikwira hose, kandi ibyago byo kwandura mu bantu no ku nyamaswa biri hejuru [1-2].
Umuyoboro
| Umuryango | Hev nucleic nucleic |
| Rox | Kugenzura imbere |
Tekinike
| Ububiko | ≤-18 ℃ |
| Imibereho-Ubuzima | Amezi 12 |
| Ubwoko bw'ikigereranyo | Umuhogo |
| Tt | ≤38 |
| CV | ≤5.0% |
| Lod | Kopi 500 / μl |
| Umwihariko | Hepatite E virusi (Hev) ni virusi ya RNA itera ibibazo byubuzima bwisi. Ifite intera ndende kandi ifite umutungo wo kwambuka interspetions. Nimwe mubintu byingenzi bya zoonotic byingenzi kandi bikagirira nabi abantu ninyamaswa. Hev yanduzwa cyane cyane binyuze muri kwanduza imyenda-ocal-ocal, kandi irashobora no kwandura ihagaritse binyuze mu nsoro cyangwa amaraso. Muri bo, mu nzira yanduye, Hev-wanduye amazi n'ibiryo bikwira hose, kandi ibyago byo kwandura mu bantu no ku nyamaswa biri hejuru [1-2]. |
| Ibikoresho bisabwa | Gukoresha Biosystems 7500 Igihe cya PCR Gushyira mubikorwa biosystems 7500 byihuse-igihe cya sisitemu ya PCR Quartudio®5 sisitemu nyayo PCR Slan-96p Sisitemu NYAKURI PCR (Tekinoroji yubuvuzi Cology Co, Ltd.) Umucyo®480 Igihe Cyigihe cya PCR Umurongo 9600 wongeyeho sisitemu yigihe cya PCR (FQD-96a, Hangzhou tekinoroji yibinyabuzima) Ma-6000 nyayo-nyayo Umwanya wa Cyrmal Cycler (Suzhou Morlary Co, Ltd.) Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Ihitamo 1
Macro & Micro-Ikizamini Rusange ADN / RNA Kit (HNAT-3017-50, hwts-3017-48, Umukino wa Macro & Micro & Micro-Ikizamini Automatic Automatic Automatic, Hwts-3006b). Igomba gukurwa ukurikije amabwiriza. Umubumbe w'ikirenga usabwa ni 80μl.
Ihitamo 2
Vianamp Virus ADN / RNA Kit (Ydp315-R) Yakozwe na Tiangen biotech (Beijing) Co., Ltd. Bikwiye gushyirwa mukuri ukurikije amabwiriza. Igitabo cyitegererezo cyakuweho ni 140μl. Umubumbe wa elldition wasabwe ni 60μl.v
