Virusi ya Hepatite B ADN ya Quantitative Fluorescence
Izina RY'IGICURUZWA
HWTS-HP015 Virusi ya Hepatite B virusi ADN Igipimo cyo gusuzuma indwara ya Fluorescence (Fluorescence PCR)
Epidemiologiya
Indwara ya Hepatite B ni indwara iterwa na virusi ya hepatite B (HBV), irangwa ahanini no gukomeretsa umwijima, kandi ishobora kwangiza ingingo nyinshi.Abarwayi ba Hepatite B bagaragara mubuvuzi nkumunaniro, kubura ubushake bwo kurya, kuruhande rwo hasi cyangwa kuribwa muri rusange, na hepatomegaly kubera imikorere mibi yumwijima.Batanu ku ijana by'abantu banduye bakuze na 95% by'abantu banduye bahagaze ntibashobora gukuraho neza HBV, bikaviramo kwandura virusi, kandi indwara zimwe na zimwe zidakira amaherezo zikavamo umwijima cirrhose na kanseri ya hepatocellular.[1-4].
Umuyoboro
| FAM | HBV-ADN |
| ROX | Igenzura ryimbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | serumu nshya 、 Plasma |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 5 IU / mL |
| Umwihariko | Ibisubizo byihariye byerekana ko ibibazo 50 byose byubuzima bwiza bwa HBV ADN ya serumu mbi;ibisubizo by'ibizamini bya cross-reactivite byerekana ko nta reaction iri hagati yiki gikoresho nizindi virusi (HAV, HCV, DFV, VIH) kugirango hamenyekane aside nucleic hamwe namaraso, hamwe na genomuntu. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe-PCR Sisitemu Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR SLAN-96P Sisitemu nyayo-PCR Sisitemu (Hongshi Medical Technology Co., Ltd.) UmucyoCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu Yigihe-PCR yo Kumenya (FQD-96A, Ikoranabuhanga rya Hangzhou Bioer) MA-6000 Igihe Cyuzuye Cyumubare Wumukino Wamagare (Suzhou Molarray Co., Ltd.) BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Ihitamo 1.
Macro & Micro-Ikizamini rusange ADN / RNA Kit (HWTS-3017) na Macro & Micro-Ikizamini cyikora Nucleic Acide ikuramo (HWTS-3006C, HWTS-3006B).Gukuramo bigomba gukorwa ukurikije igitabo gikubiyemo amabwiriza, urugero rwakuweho ni 300μL, naho urugero rwo gukuraho ni 70μL.
