Virusi ya Hepatite B ADN ifite ubwinshi bw'urumuri
Izina ry'igicuruzwa
Kit yo gusuzuma ubwinshi bwa virusi ya HWTS-HP015 Hepatite B ADN (Fluorescence PCR)
Ubumenyi bw'indwara
Hepatite B ni indwara iterwa na virusi ya hepatite B (HBV), irangwa ahanini n'ibisebe byo mu mwijima, kandi ishobora kwangiza ingingo nyinshi. Abarwayi ba hepatite B bagaragara nk'umunaniro, kubura ubushake bwo kurya, kubyimba kw'inyuma cyangwa muri rusange, no gucika intege bitewe n'imikorere mibi y'umwijima. Batanu ku ijana by'abantu bakuru banduye na 95% by'abantu banduye bahagaze ntibashobora gukiza HBV neza, bigatuma bakomeza kwandura virusi, kandi zimwe mu ndwara zidakira amaherezo zigahinduka cirrhose y'umwijima na kanseri y'umwijima.[1-4].
Umuyoboro
| FAM | HBV-DNA |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | serum nshya, plasma |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 5 IU/mL |
| Ubwihariye | Ibisubizo by’imiterere yihariye bigaragaza ko abantu 50 bose banduye HBV ADN mu maraso yabo banduye nta virusi itera SIDA bafite; ibisubizo by’ibizamini bya cross-reactivity bigaragaza ko nta mpungenge hagati y’iyi kit n’izindi virusi (HAV, HCV, DFV, VIH) kugira ngo hamenyekane aside nucleic hakoreshejwe ingero z’amaraso, na genome z’abantu. |
| Ibikoresho Bikoreshwa | Sisitemu ya PCR ikoreshwa mu gihe nyacyo ya 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 Sisitemu za PCR za QuantStudio®5 mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.) Sisitemu ya LightCycler®480 Real-Time PCR Sisitemu yo Gupima PCR ya LineGene 9600 Plus mu gihe nyacyo (FQD-96A, ikoranabuhanga rya Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermocycler (Suzhou Molarray Co., Ltd.) Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Reagent isabwa gukurwamo: Macro & Micro-Test Virus DNA/RNA Kit (HWTS-3017) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ya Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Gukurwamo bigomba gukorwa hakurikijwe amabwiriza, ingano y'icyitegererezo cyakuwemo ni 300μL, naho ingano y'icyitegererezo isabwa gukurwamo ni 70μL.
