Virusi ya Hepatite A
Izina ry'igicuruzwa
Kit yo Gupima Aside Nucleic ya HWTS-HP005 Hepatite A Virus (Fluorescence PCR)
Ubumenyi bw'indwara
Virusi ya Hepatite A (HAV) ni yo mpamvu nyamukuru itera hepatitis iterwa na virusi. Iyi virusi ni virusi ya RNA ifite imiterere imwe kandi iri mu bwoko bwa Hepadnaviruse bwo mu muryango wa Picornaviridae. Virusi ya Hepatite A, ahanini yandurira mu nzira y’amafi yo mu kanwa, irwanya ubushyuhe, aside, n’ibintu byinshi biyungurura umubiri, ishobora kubaho igihe kirekire mu binyamisogwe, mu mazi, mu butaka, cyangwa mu mazi yo mu nyanja [1-3]. Yandurira mu kugira ibiryo cyangwa amazi byanduye, cyangwa byandurira mu buryo butaziguye bivuye ku muntu ku wundi. Ibiribwa bifitanye isano na HAV birimo oysters na clams, strawberries, raspberries, blueberries, amadeti, imboga z’icyatsi kibisi, n’inyanya zumye [4-6].
Umuyoboro
| FAM | aside nucleike ya HAV |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amazi: Amezi 9, Lyophilized: Amezi 12 |
| Ubwoko bw'ingero | Serum/intebe |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Kopi 2/μL |
| Ubwihariye | Koresha ibikoresho byo gupima izindi virusi za hepatite nka hepatite B, C, D, E, enterovirus 71, virusi ya coxsackie, virusi ya Epstein-Barr, virusi ya norovirus, virusi itera SIDA na genome y'umuntu. Nta buryo bwo guhuza ubwoko bw'indwara. |
| Ibikoresho Bikoreshwa | Sisitemu ya PCR ikoreshwa mu gihe nyacyo ya 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.) Sisitemu ya LightCycler®480 Real-Time PCR Sisitemu yo Gupima PCR ya LineGene 9600 Plus mu gihe nyacyo (FQD-96A, ikoranabuhanga rya Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Ingero za serum
Amahitamo ya 1.
Kit ya Macro & Micro-Test General ADN/RNA (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Igomba gukurwamo hakurikijwe amabwiriza. Ingano ya elution isabwa ni 80µL.
Amahitamo ya 2.
TIANamp Virus DNA/RNA Kit (YDP315-R) yakozwe na Tiangen Biotech (Beijing) Co., Ltd. Igomba gukurwamo hakurikijwe amabwiriza. Ingano y'icyitegererezo cyakuwemo ni 140μL. Ingano y'icyitegererezo cyatanzwe ni 60µL.
2.Ingero z'intebe
Kit ya Macro & Micro-Test General ADN/RNA (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Igomba gukurwamo hakurikijwe amabwiriza. Ingano ya elution isabwa ni 80µL.
