Gukonjesha Zayire na Sudani Ebolavirus Nucleic Acide
Izina ryibicuruzwa
HWTS-FE035-Gukonjesha Zayire na Sudani Ebolavirus Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Ebolavirus ni iya Filoviridae, ikaba ari virusi ya RNA itagabanijwe. Virusi ni filime ndende ifite impuzandengo ya virusi ya 1000nm na diameter ya 100nm. Indwara ya Ebolavirus ni RNA itagabanijwe kandi ifite ubunini bwa 18.9kb, ikubiyemo poroteyine 7 zubaka na poroteyine 1 idafite imiterere. Ebolavirus irashobora kugabanywamo ubwoko nka Zaire, Sudani, Bundibugyo, Ishyamba rya Tai na Reston. Muri bo, ubwoko bwa Zaire n'ubwoko bwa Sudani byavuzwe ko bitera abantu benshi bapfa bazize indwara. EHF (Indwara ya Ebola Hemorrhagic) ni indwara ikabije ya hemorhagie yanduye iterwa na Ebolavirus. Abantu bandura cyane cyane guhura namazi yumubiri, gusohora no gusohora abarwayi cyangwa inyamaswa zanduye, kandi kwigaragaza kwa muganga usanga ahanini byerekana umuriro, kuva amaraso no kwangirika kwingingo nyinshi. EHF ifite impfu nyinshi zingana na 50% -90%. Kugeza ubu, uburyo bwo gusuzuma Ebolavirus ni ibizamini bya laboratoire, harimo ibintu bibiri: gutahura etiologiya no kumenya serologiya. Kumenya indwara ya Etiologiya harimo kumenya virusi ya virusi mu byitegererezo by'amaraso na ELISA, kumenya aside nucleic hakoreshejwe uburyo bwo kongera imbaraga nka RT-PCR, n'ibindi, no gukoresha selile Vero, Hela, n'ibindi mu gukumira virusi n'umuco. Gutahura serologiya bikubiyemo kumenya antibodiyumu za Serumu zihariye zifata ELISA, no kumenya serumu yihariye ya IgG na ELISA, immunofluorescence, nibindi.
Ibipimo bya tekiniki
Ububiko | ≤30 ℃ |
Ubuzima bwa Shelf | Amezi 12 |
Ubwoko bw'icyitegererezo | serumu, plasma |
CV | ≤5.0% |
LoD | Amakopi 500 / μL |
Ibikoresho bikoreshwa | Bikenewe kugirango wandike I detection reagent: Ikoreshwa rya Biosystems 7500-Igihe-PCR Sisitemu, QuantStudio®5 Sisitemu nyayo-PCR Sisitemu, SLAN-96P Sisitemu nyayo-PCR (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Yongeyeho Sisitemu Yukuri-PCR Yerekana (FQD-96A, Ikoranabuhanga rya Hangzhou Bioer), MA-6000 Igihe Cyuzuye Cyumubyigano Wumukino (Suzhou Molarray Co., Ltd.), BioRad CFX96 Sisitemu Yigihe-PCR Sisitemu, BioRad CFX Opus 96 Sisitemu Yigihe-PCR. Birakoreshwa mubwoko bwa II bwo gutahura reagent: EudemonTMAIO800 (HWTS-EQ007) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Urujya n'uruza rw'akazi
Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acide (HWTS-3006). Gukuramo bigomba gukorwa ukurikije amabwiriza, naho urugero rwakuweho ni 200μL naho icyifuzo cyo gukuraho ni 80μL.