Chlamydia Trachomatis yumye mu bukonje
Izina ry'igicuruzwa
HWTS-UR032C/D-Kit yo Gupima Aside ya Chlamydia Trachomatis Nucleic (Enzymatic Probe Isothermal Amplification) yumye mu bukonje
Ubumenyi bw'indwara
Chlamydia trachomatis (CT) ni ubwoko bwa mikorobe ya prokaryotic ikunze kuba mu turemangingo twa eukaryotic gusa.[1]Chlamydia trachomatis igabanyijemo serotypes za AK hakurikijwe uburyo bwa serotype. Indwara zo mu nzira y'ubugimbi/urogenital ziterwa ahanini na serotypes za trachoma biological variant DK, naho abagabo bakunze kugaragara nk'indwara ya urethritis, ishobora kugabanuka hatabayeho kuvurwa, ariko inyinshi muri zo ziba zidakira, rimwe na rimwe zikongera ubukana, kandi zishobora kuvangwa na epididymitis, proctitis, nibindi.[2]Abagore bashobora guterwa n'indwara yo mu muhogo, cervicitis, n'ibindi, ndetse n'izindi ngorane zikomeye za salpingitis.[3].
Umuyoboro
| FAM | Chlamydia trachomatis (CT) |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤30℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Igitambaro cyo mu mura cy'umugore Igitambaro cyo mu muyoboro w'inkari cy'abagabo Inkari z'abagabo |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | Kopi 400/mL |
| Ubwihariye | Nta buryo bwo guhuza iyi kit n'izindi ndwara zitera indwara zo mu myanya ndangagitsina nka virusi ya Human papillomavirus yo mu bwoko bwa 16 ifite ibyago byinshi, virusi ya Human papillomavirus yo mu bwoko bwa 18, virusi ya Herpes simplex yo mu bwoko bwa Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Staphylococcus epidermidis, Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, virusi itera ubudahangarwa bw'umubiri bw'umuntu, Lactobacillus casei na ADN ya genome y'umuntu, n'ibindi. |
| Ibikoresho Bikoreshwa | Sisitemu ya PCR ikoreshwa mu gihe nyacyo ya 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.) LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu yo Gupima PCR ya LineGene 9600 Plus mu gihe nyacyo (FQD-96A, ikoranabuhanga rya Hangzhou Bioer) MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 Real-Time PCR na Sisitemu ya BioRad CFX Opus 96 Real-Time PCR Sisitemu yo Gupima Ubushyuhe bw'Izuba mu Gihe Nyacyo (Amp Real-Time Fluorescence Isothermal Detection System) yoroshye(cyangwa seHWTS-1600). |
Urugendo rw'akazi
Amahitamo ya 1.
Ikizamini cya Macro & Micro-Test Release Reagent (HWTS-3005-8). Gukuramo bigomba gukorwa hakurikijwe amabwiriza ya IFU. Shyira ADN y'icyitegererezo yakuwemo n'ikizamini cya release reagent mu cyuma gipima reaction buffer hanyuma ugerageze ku gikoresho, bitabaye ibyo ibizamini byakuwemo bigomba kubikwa kuri 2-8℃ mu gihe kitarenze amasaha 24.
Amahitamo ya 2.
Kit rusange ya ADN/RNA ya Macro na Micro-Test (HWTS-301)7-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Gukuramo bigomba gukorwa hakurikijwe IFU, kandi ingano ya elution isabwa ni 80μL. ADN y'icyitegererezo ikurwamo hakoreshejwe uburyo bwa magnetic bead ishyushywa kuri 95°C mu gihe cy'iminota 3 hanyuma igahita yogezwa mu rubura mu gihe cy'iminota 2. Shyira ADN y'icyitegererezo yatunganyijwe mu cyuma gipima reaction buffer hanyuma igeragezwa ku gikoresho cyangwa ingero zatunganyijwe rigomba kubikwa munsi ya -18°C mu gihe kitarenze amezi 4. Umubare w'inshuro zisubirwamo zo gukonjesha no gushonga ntugomba kurenza iminota 4.
