Gukonjesha-byumye 11 Ubwoko bwa Pathogene Yubuhumekero Nucleic Acide
Izina ryibicuruzwa
HWTS-RT190 -Gukonjesha-gukama-Gukonjesha-gukama 11 Ubwoko bwa Pathogens Yubuhumekero Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Indwara z'ubuhumekero ni indwara ikomeye ibangamira cyane ubuzima bw'abantu. Ubushakashatsi bwerekanye ko indwara nyinshi zandurira mu myanya y'ubuhumekero ziterwa na bagiteri na / cyangwa virusi ziterwa na virusi zanduza nyirubwite, bigatuma ubukana bw'indwara bwiyongera cyangwa se n'urupfu. Kubwibyo, kumenya icyorezo gishobora gutanga ubuvuzi bugamije kandi bikazamura ubuzima bwumurwayi [1,2]. Nyamara, uburyo gakondo bwo kumenya indwara ziterwa nubuhumekero harimo kwisuzumisha microscopique, umuco wa bagiteri, no gusuzuma immunologiya. Ubu buryo buragoye, butwara igihe, busaba tekiniki, kandi bufite sensibilité nkeya. Byongeye kandi, ntibashobora kumenya virusi nyinshi murugero rumwe, bigatuma bigora guha abaganga kwisuzumisha ryukuri. Kubera iyo mpamvu, imiti myinshi iracyari mu rwego rwo gufata imiti, ntabwo yihutisha ukwezi kwa bagiteri gusa, ahubwo inagira ingaruka ku gusuzuma ku gihe [3]. Indwara ya grippe Haemophilus, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bordetella parapertussis, Mycoplasma parapertussis, Mycoplasma pneertia ni indwara zitera indwara zandurira mu myanya y'ubuhumekero nosocomial [4,5]. Iki gikoresho cyo gupima kimenyekanisha kandi kigaragaza acide nucleic yihariye ya virusi yavuzwe haruguru ku bantu bafite ibimenyetso nibimenyetso byindwara zubuhumekero, ikanabihuza nibindi bisubizo bya laboratoire kugirango ifashe mugupima indwara zandurira mu myanya y'ubuhumekero.
Ibipimo bya tekiniki
| Ububiko | 2-30 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | Umuhogo |
| Ct | ≤33 |
| CV | <5.0% |
| LoD | LoD y'ibikoresho bya Klebsiella pneumoniae ni 500 CFU / mL; LoD ya Streptococcus pneumoniae ni 500 CFU / mL; LoD ya grippe Haemophilus ni 1000 CFU / mL; LoD ya Pseudomonas aeruginosa ni 500 CFU / mL; LoD ya Acinetobacter baumannii ni 500 CFU / mL; LoD ya Stenotrophomonas maltophilia ni 1000 CFU / mL; LoD ya Bordetella pertussis ni 500 CFU / mL; LoD ya Bordetella parapertussis ni 500 CFU / mL; LoD ya Mycoplasma pneumoniae ni kopi 200 / mL; LoD ya Legionella pneumophila ni 1000 CFU / mL; LoD ya Chlamydia pneumoniae ni kopi 200 / mL. |
| Umwihariko | Nta reaction yambukiranya hagati yiki gikoresho nizindi ndwara ziterwa nubuhumekero zisanzwe hanze yikimenyetso cyo gupima, urugero: Staphylococcus aureus, epidermidis ya Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella oxytoca, Streptococcus Listeria monocytogène, Acinetobacter junii, Haemophilus parainfluenzae, Legionella dumov, Enterobacter aerogenes, Haemophilus haemolyticus, Streptococcus salivarius, Neisseria meningitidis, Mycobacterium tuberculose, Influenza A virusi, Influenza A virusi Aspergillus fumigatus, Candida glabrata, na Candida tropicalis. |
| Ibikoresho bikoreshwa | Ubwoko I: Ikoreshwa ryibinyabuzima 7500 Byukuri-Igihe cya PCR Sisitemu, Ikoreshwa rya Biosystems 7500 Byihuse-Igihe Cyuzuye PCR, QuantStudio®Sisitemu 5-Igihe nyacyo PCR, SLAN-96P Sisitemu nyayo-PCR (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Sisitemu nyayo-PCR, LineGene 9600 Yongeyeho Sisitemu Yigihe Cyigihe cyo Kumenya PCR (FQD-96A, Ikoranabuhanga rya Hangzhou Bioer), MA-6000 Igihe Cyuzuye Cyumubyigano Cyamashanyarazi (Suzhou Molarray Co., Ltd.), BioRad CFX96 Sisitemu Yigihe-PCR, BioRad CFX Opus 96 Sisitemu Yigihe-PCR. Ubwoko bwa II: EudemonTMAIO800 (HWTS-EQ007) na Jiangsu Macro & Micro-Test Med-Tech Co. Ltd. |
Urujya n'uruza rw'akazi
Ubwoko bwa I: Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3017) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd irasabwa gukuramo icyitegererezo kandi hakurikiraho ingamba zikurikira zigomba gukorwa muburyo bukurikira.
