Enterovirus Universal, EV71 na CoxA16
Izina ryibicuruzwa
HWTS-EV026B-Enterovirus Universal, EV71 na CoxA16 Nucleic Acide Detection Kit (Fluorescence PCR)
HWTS-EV020Y / Z.-Feroze yumye Enterovirus Universal, EV71 na CoxA16 Nucleic Acide Detection Kit (Fluorescence PCR)
Icyemezo
CE / MDA （HWTS-EV026）
Epidemiologiya
Indwara yo mu kanwa (HFMD) ni indwara ikunze kwandura abana. Bikunze kugaragara cyane ku bana bari munsi yimyaka 5, kandi birashobora gutera herpes kumaboko, ibirenge, umunwa nibindi bice, kandi umubare muto wabana urashobora gutera ingorane nka myocarditis, indurwe yimpyiko, aseptic meningoencephalitis, nibindi.
Kugeza ubu, serotypes 108 za enterovirusi zabonetse, zigabanyijemo amatsinda ane: A, B, C na D. Enterovirus zitera HFMD ziratandukanye, ariko enterovirus 71 (EV71) na coxsackievirus A16 (CoxA16) nizo zikunze kugaragara kandi usibye HFMD, zishobora gutera ibibazo bikomeye bya sisitemu yo mu bwoko bwa paracchalite, ence.
Umuyoboro
| FAM | Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Kugenzura imbere |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18 ℃ Mu mwijimaLyophilisation: ≤30 ℃ |
| Ubuzima bwa Shelf | Amazi: amezi 9Lyophilisation: amezi 12 |
| Ubwoko bw'icyitegererezo | Umuhogo wa swab sample, Herpes fluid |
| Ct | ≤38 |
| CV | ≤5.0 ％ |
| LoD | 500Copi / mL |
| Ibikoresho bikoreshwa | Irashobora guhuza ibikoresho nyamukuru bya fluorescent PCR kumasoko.ABI 7500 Sisitemu nyayo-PCR ABI 7500 Byihuse-Igihe Cyuzuye PCR SLAN-96P Sisitemu nyayo-PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR UmucyoCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho-Igihe-Cyukuri cya PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Igisubizo cya PCR
Gusabwa gukuramo reagent: Macro & Micro-Test Virus ADN / RNA Kit (HWTS-3017) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-EQ011)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Gukuramo bigomba gukorwa ukurikije igitabo gikubiyemo amabwiriza. Ingano yicyitegererezo yakuweho ni 200μL, naho icyifuzo cyo gukuraho ni 80μL.
Basabwe gukuramo reagent: Macro & Micro-Ikigereranyo Cyitegererezo cyo Gusohora Reagent (HWTS-3005-8). Gukuramo bigomba gukorwa ukurikije imfashanyigisho. Ingero zo gukuramo ni oropharyngeal swabs cyangwa herpes fluid sample yabarwayi bakusanyirije kurubuga. Ongeramo swabs yakusanyirijwe muri Macro & Micro-Test Sample Release Reagent, vortex hanyuma uvange neza, shyira mubushyuhe bwicyumba muminota 5, fata hanyuma uhindure hanyuma uvange neza kugirango ubone RNA ya buri sample.
Gusabwa gukuramo reagent: QIAamp Viral RNA Mini Kit (52904) na QIAGEN cyangwa Gukuramo Acide Nucleic Acide cyangwa Isukura Reagent (YDP315-R). Gukuramo bigomba gukorwa hakurikijwe igitabo gikubiyemo amabwiriza.
