Enterovirus kwisi yose, Ev71 na Coxa16
Izina ry'ibicuruzwa
Hwts-ev026B-Enterovirus Isi Yose, Ev71 na Coxa16 Acide Gutahura Acide Gutahura (Fluorescence PCR)
Hwts-ev020Y / Z-Iburyo bwa Enterovirus kwisi yose, ev71 na Coxa16 Acide Gutahura Acide Gutahura (Fluorescence PCR)
Icyemezo
CE / MDA (HWTS-EV026)
Epidemiology
Indwara y'umunwa w'intoki (HFMD) ni indwara isanzwe yanduye mu bana. Ahantu ahaboneka mubana bari munsi yimyaka 5, kandi barashobora gutera umurage, umunwa, kandi umubare muto wa Edema, kandi umubare muto wa Myokartililitis, nibindi byinshi hamwe nabana bakomeye Indwara zangiritse vuba kandi zikunda urupfu niba zidafashwe bidatinze.
Kugeza ubu, 108 Serotypes ya Enteroviriye, igabanijwemo amatsinda ane: A, B, B na D. En171) na Coxsackievirus A16 (CoxsaCracUsvievirus A16 (Coxa16) ni rusange kandi muri Kwiyongera kuri HFMD, birashobora gutera ibibazo bikomeye bya sisitemu yo hagati ya sisitemu nka meningite, encephalitis, na ubumuga bukabije.
Umuyoboro
| Umuryango | Enterovir |
| Vic (hex) | Coxa16 |
| Rox | Ev71 |
| Cy5 | Kugenzura imbere |
Tekinike
| Ububiko | Amazi: ≤-18 ℃ mu mwijimaLyophilisation: ≤30 ℃ |
| Imibereho-Ubuzima | Amazi: amezi 9Lyophilisation: amezi 12 |
| Ubwoko bw'ikigereranyo | Umuhogo Swab Icyitegererezo, Herpes Fluid |
| Ct | ≤38 |
| CV | ≤5.0% |
| Lod | 500copies / ml |
| Ibikoresho bisabwa | Irashobora guhuza ibikoresho nyamukuru bya PCR ku isoko.Abi 7500 Igihe Cyigihe cya PCR Abi 7500 byihuse-sisitemu ya PCR Scan-96p sisitemu nyayo pcr sisitemu Quartudio®5 sisitemu nyayo PCR Umucyo®480 Igihe Cyigihe cya PCR Linegene 9600 wongeyeho sisitemu nyayo ya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Igisubizo cya PCR
Basabwe gukuramo Reagent: Macro & Micro-Ikizamini cya virusi ya virusi (Hnat-3017) Co., Ltd. Gukuramo bigomba gukorwa hakurikijwe igitabo cyigisha amabwiriza. Igitabo cyitegererezo cyakuweho ni 200μl, hamwe nubunini bwabigenewe ni 80μl.
Basabwe gukuramo reagent: Macro & Micro-Ikizamini Icyitegererezo cya Regent (HWTS-3005-8). Gukuramo bigomba gukorerwa ukurikije igitabo cyigisha amabwiriza. Gukuramo ingero ni swab ya oropharyngeal cyangwa herpes yamazi yintangarugero yabarwayi bakusanyije kurubuga. Ongeramo Swab yakusanyirijwe muri Macro & Micro-Ikizamini Icyitegererezo cya Rero, Vortex hanyuma uvange neza, shyira ku bushyuhe bwo mucyumba, fata hanyuma uvange neza kugirango ubone RNA ya buri cyitegererezo.
Basabwe gukuramo reagent: Qiaip viral rna mini kit (52904) na Qiagen cyangwa indude ya acide cyangwa kweza reagent (ydp315-r). Gukuramo bigomba gukorwa muburyo bukomeye.
