Enterovirus Universal, EV71 na CoxA16
Izina ry'igicuruzwa
HWTS-EV026B-Enterovirus Universal, EV71 na CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
HWTS-EV020Y/Z-Ikigega cyo gupima aside ya fluorescence cyumye cya Enterovirus Universal, EV71 na CoxA16 (Fluorescence PCR)
Icyemezo
CE/MDA（HWTS-EV026）
Ubumenyi bw'indwara
Indwara y'ikirenge n'intoki (HFMD) ni indwara ikunze kwandura mu bana. Ikunze kugaragara cyane mu bana bari munsi y'imyaka 5, kandi ishobora gutera herpes ku maboko, ku birenge, ku kanwa no ku bindi bice by'umubiri, kandi umubare muto w'abana ushobora gutera ingorane nka myocarditis, kubyimba ibihaha, meningoencephalitis idaseptic, n'ibindi. Abana bafite indwara zikomeye barushaho kwangirika vuba kandi bashobora gupfa iyo badahawe umuti vuba.
Ubu, habonetse ubwoko 108 bwa serovirusi za enterovirusi, zigabanyemo amatsinda ane: A, B, C na D. Enterovirusi zitera HFMD ziratandukanye, ariko enterovirusi 71 (EV71) na coxsackievirusi A16 (CoxA16) nizo zikunze kugaragara cyane kandi uretse HFMD, zishobora gutera ingorane zikomeye z’imitsi yo hagati nka meningitis, encephalitis, na paralysis ikabije.
Umuyoboro
| FAM | Virusi ya Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18℃ Mu mwijimaLyophilization: ≤30℃ |
| Igihe cyo kumara | Amazi: Amezi 9Lyophilization: amezi 12 |
| Ubwoko bw'ingero | Icyitegererezo cy'umuhogo, Herpes fluid |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | Kopi 500/mL |
| Ibikoresho Bikoreshwa | Ishobora guhuza n'ibikoresho bya PCR bikoreshwa mu gupima urumuri biri ku isoko.Sisitemu za PCR za ABI 7500 mu gihe nyacyo Sisitemu za PCR za ABI 7500 zihuta cyane mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo Sisitemu za PCR za QuantStudio®5 mu gihe nyacyo Sisitemu za PCR za LightCycler®480 mu gihe nyacyo Sisitemu zo Gusuzuma za LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Igisubizo cya PCR cyose
Reagent isabwa gukurwamo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ya Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Gukurwamo bigomba gukorwa hakurikijwe amabwiriza. Ingano y'icyitegererezo cyakuwemo ni 200μL, naho ingano y'icyitegererezo isabwa gukurwamo ni 80μL.
Ikoreshwa mu gukuramo umuti risabwa: Ikoreshwa mu gukuramo umuti mu buryo bwa Macro & Micro-Test (HWTS-3005-8). Gukuramo umuti bigomba gukorwa hakurikijwe amabwiriza. Ingero zo gukuramo umuti ni ingero z'amazi ya oropharyngeal cyangwa ingero z'amazi ya herpes y'abarwayi bakusanyijwe aho hantu. Shyira ingero zakusanyijwe mu buryo butaziguye kuri Macro & Micro-Test Sample Release Reagent, vortex hanyuma uvange neza, ushyire ku bushyuhe bw'icyumba mu gihe cy'iminota 5, ukuremo hanyuma uhindure umuti hanyuma uvange neza kugira ngo haboneke RNA ya buri ngero.
Uburyo bwo gukuramo ibintu busabwa: QIAamp Viral RNA Mini Kit (52904) hakoreshejwe QIAGEN cyangwa Nucleic Acid Extraction cyangwa Purification Reagent (YDP315-R). Gukuramo ibintu bigomba gukorwa hakurikijwe amabwiriza y’amabwiriza.
