Enterovirus 71 (EV71)
Izina RY'IGICURUZWA
HWTS-EV003- Enterovirus 71 (EV71) Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Indwara yo mu kanwa ni indwara yandura iterwa na enterovirusi (EV).Kugeza ubu, ubwoko 108 bwa serotypes ya enterovirusi bwabonetse, bugabanijwe mu matsinda ane: A, B, C na D. Muri bo, enterovirus EV71 na CoxA16 nizo zitera indwara nyamukuru.Indwara ahanini igaragara ku bana bari munsi yimyaka 5, kandi irashobora gutera herpes kumaboko, ibirenge, umunwa nibindi bice.Umubare muto wabana bazagira ibibazo nka myocarditis, edmonary edema, na aseptic meningoencephalitis.
Umuyoboro
| FAM | EV71 |
| ROX | Igenzura ryimbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 9 |
| Ubwoko bw'icyitegererezo | Oropharyngeal swab ，Herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500Copi / mL |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho-Igihe-Cyukuri cya PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Ihitamo 1.
Gusabwa gukuramo reagent: Macro & Micro-Ikigereranyo Cyitegererezo cyo Kurekura Reagent (HWTS-3005-8), kandi gukuramo bigomba gukorwa ukurikije amabwiriza yo gukoresha.Ibyitegererezo byakuweho ni oropharyngeal swabs cyangwa herpes fluid sample yabarwayi bakusanyirije kurubuga.Ongeramo swabs yakusanyirijwe muri Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) itaziguye, vortex hanyuma uvange neza, shyira mubushyuhe bwicyumba muminota 5, ubikuremo hanyuma uhindure kuvanga neza, kugirango ubone RNA ya buri cyitegererezo.
Icya 2.
Gusabwa gukuramo reagent: Macro & Micro-Ikizamini Rusange ADN / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa na Macro & Micro-Ikizamini Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., kandi kuyikuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha.Icyitegererezo cyakuweho ni 200µL, naho icyifuzo cyo gukuraho ni 80µL.
Ihitamo3.
Gusabwa gukuramo reagent: QIAamp Viral RNA Mini Kit (52904) na QIAGEN cyangwa TIANamp Virus ADN / RNA Kit (YDP315-R), kandi kuyikuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha.Icyitegererezo cyakuweho ni 140μL, naho icyifuzo cyo gukuraho ni 60µL.
