Enterovirus 71 (Ev71)
Izina ry'ibicuruzwa
Hwts-ev003- Enterovirus 71 (Ev71) Acide ya Acic Gutahura KUNCLECH (Fluorescence PCR)
Epidemiology
Indwara y'umunwa y'intoki ni indwara yanduza iterwa na Enterovirsus (EV). Kugeza ubu, ubwoko 108 bw'ubwoko bwa Enterovirpes bwabonetse, bugabanyijemo amatsinda ane: A, B, B, B. Muri bo, Enterovirus Ev71 na Coxa16 ni pathogens nyamukuru. Indwara ibaho mu bana bari munsi yimyaka 5, kandi irashobora gutera ibya herpes kumaboko, ibirenge, umunwa, no mubindi bice. Umubare muto w'abana uzamura ingorane nka Myocardite, Pulmone Edema, na AsEptic MenoistFlitilis.
Umuyoboro
| Umuryango | Ev71 |
| Rox | Kugenzura imbere |
Tekinike
| Ububiko | ≤-18 ℃ |
| Imibereho-Ubuzima | Amezi 9 |
| Ubwoko bw'ikigereranyo | Sropheryngeal Swab,Herpes |
| Ct | ≤35 |
| CV | <5.0% |
| Lod | 500copies / ml |
| Ibikoresho bisabwa | Gushyira mu bikorwa biosystems 7500 igihe cya sisitemu ya PCR Gushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Umutobe®5-igihe nyacyo PCR sisitemu Scan-96p sisitemu nyayo pcr sisitemu Umucyo®480 nyamara igihe cya PCR Linegene 9600 wongeyeho sisitemu nyayo ya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Ihitamo rya 1.
Gusaba gukuramo reagent: Macro & Micro-Ikizamini Icyitegererezo cya Regent (HWTS-3005-8), kandi gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha. Urugero rwakuweho ni swab ya oropharyngeal cyangwa herpes yamazi yintangarugero yakusanyije kurubuga. Ongeramo Swib yakusanyijwe muri Macro & Micro-Ikizamini Icyitegererezo cya Regent (HWTS-3005-8) mu buryo butaziguye, shyira hejuru y'iminota 5, shyira mu cyumba cyo kuvanga neza, kugirango ubone RNA ya buri cyitegererezo.
IHitamo 2.
Basabwe gukuramo Reagent: Macro-Ikizamini Rusange ADN / RNA Kit (HWTS-3017-50, hwts-3017-48, HWTS-3017-96) (Birashobora gukoreshwa hamwe na macro & micro-ikizamini Automatic Autocctor ya acide (HWTS-3006C, HWTS-3006B)) na Jiagsu Macro & Micro-Ikizamini Med-Tech Co., Ltd., kandi gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha. Igitabo cyitegererezo cyakuweho ni 200μl, hamwe nubunini bwabigenewe ni 80μl.
Ihitamo3.
Gusaba gukuramo reagent: Qiaip viral rna mini kit (52904) na Qiagen ya Quagen cyangwa tinamp am / rna kit (ydp315-r) Igitabo cyitegererezo cyakuweho ni 140μl, kandi umubumbe wabisabwa ni 60μl.
