Virusi ya Enterovirusi 71 (EV71)
Izina ry'igicuruzwa
HWTS-EV003- Enterovirus 71 (EV71) Kit yo Gupima Acide Nucleic (Fluorescence PCR)
Ubumenyi bw'indwara
Indwara yo mu kanwa k'intoki ni indwara yandura iterwa na virusi za enterovirus (EV). Ubu, hamaze kuboneka ubwoko 108 bwa virusi za enterovirus, zigabanyijemo amatsinda ane: A, B, C na D. Muri yo, virusi ya enterovirus EV71 na CoxA16 ni zo ndwara nyamukuru zitera. Iyi ndwara ikunze kugaragara mu bana bari munsi y'imyaka 5, kandi ishobora gutera herpes ku biganza, ku birenge, ku kanwa no ku bindi bice. Abana bake bazahura n'ibibazo nka myocarditis, kubyimba ibihaha, na meningoencephalitis idaseptic.
Umuyoboro
| FAM | EV71 |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 9 |
| Ubwoko bw'ingero | Agatambaro k'umuhogo,Amazi ya Herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | Kopi 500/mL |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo LightCycler®Sisitemu 480 za PCR mu gihe nyacyo Sisitemu zo Gusuzuma za LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Amahitamo ya 1.
Reagent isabwa gukurwamo: Reagent yo gukuramo icyitegererezo cya Macro & Micro-Test (HWTS-3005-8), kandi gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoreshwa. Ingero zakuwemo ni swab zo mu kanwa cyangwa ingero z'amazi ya herpes zo mu barwayi bakusanyirijwe aho hantu. Shyiramo swab zakusanyijwe muri Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) neza, uvange neza, ushyire ku bushyuhe bw'icyumba mu gihe cy'iminota 5, ubikuremo hanyuma ubihindure kugira ngo bivange neza, kugira ngo haboneke RNA ya buri gipimo.
Amahitamo ya 2.
Reagent isabwa gukurwamo: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., kandi gukurwamo bigomba gukorwa hakurikijwe amabwiriza yo gukoreshwa. Ingano y'icyitegererezo cyakuwemo ni 200µL, naho ingano y'icyitegererezo cyatanzwe ni 80µL.
Amahitamo ya 3.
Uburyo bwo gukuramo ibintu busabwa: QIAamp Viral RNA Mini Kit (52904) na QIAGEN cyangwa TIANamp Virus DNA/RNA Kit (YDP315-R), kandi gukuramo ibintu bigomba gukorwa hakurikijwe amabwiriza yo kubikoresha. Ingano y'icyitegererezo cyakuwemo ni 140μL, naho ingano y'icyitegererezo cyatanzwe ni 60μL.
