Virusi ya Dengue, Zika Virus na Chikungunya Virus Multiplex
Izina ryibicuruzwa
HWTS-FE040 Virusi ya Dengue, Virusi ya Zika na Chikungunya Virus Multiplex Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Indwara ya Dengue (DF), iterwa na virusi ya dengue (DENV), ni imwe mu ndwara zandura cyane arbovirus. Ikwirakwizwa ryayo ririmo Aedes aegypti na Aedes albopictus. DF yiganje cyane mu turere dushyuha no mu turere dushyuha. DENV ni iya flavivirus munsi ya flaviviridae, kandi irashobora gushyirwa muri serotype 4 ukurikije antigen yo hejuru. Kugaragara kwa muganga kwandura DENV ahanini birimo kubabara umutwe, umuriro, intege nke, kwaguka kwa lymph node, leukopenia nibindi, hamwe no kuva amaraso, guhungabana, gukomeretsa umwijima cyangwa no gupfa mubihe bikomeye. Mu myaka yashize, imihindagurikire y’ikirere, imijyi, iterambere ryihuse ry’ubukerarugendo n’ibindi bintu byatanze uburyo bwihuse kandi bworoshye bwo kwanduza no gukwirakwiza DF, bigatuma habaho icyorezo cy’icyorezo cya DF.
Umuyoboro
| FAM | DENV acide nucleic |
| ROX | Igenzura ryimbere |
Ibipimo bya tekiniki
| Ububiko | -18 ℃ |
| Ubuzima bwa Shelf | Amezi 9 |
| Ubwoko bw'icyitegererezo | Serumu nshya |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | Amakopi 500 / mL |
| Umwihariko | Ibisubizo by’ibizamini byerekana ko iyo bilirubine iri muri serumu itarenze 168.2μmol / ml, intungamubiri ya hemoglobine ikorwa na hemolysis ntirenza 130g / L, ubwinshi bw’amaraso ya lipide ntabwo burenga 65mmol / ml, igiteranyo cya IgG muri serumu ntikirenza 5mg / mL, nta ngaruka za virusi ya dengue, Zika. Virusi ya Hepatitis A, virusi ya Hepatitis B, virusi ya Hepatite C, virusi ya Herpes, virusi ya encephalitis yo mu burasirazuba, virusi ya Hantavirus, Bunya, virusi ya West Nile na virusi ya genomic serumu y’abantu yatoranijwe kugira ngo isuzumwe n’ibisubizo, kandi ibisubizo byerekana ko nta reaction y’umusaraba iri hagati y’iki gitabo na virusi zavuzwe haruguru. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe-PCR Sisitemu Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu nyayo-PCR Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Ihitamo 1.
TIANamp Virus ADN / RNA Kit (YDP315-R), kandi kuyikuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha. Ingano yicyitegererezo yakuweho ni 140μL, naho ingano yo gusabwa ni 60μL.
Icya 2.
Macro & Micro-Ikizamini rusange ADN / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa na Macro & Micro-Test Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B) ikorwa na Jiangsu Macro & Micro-Tech. Kuri Koresha. Ingano yicyitegererezo yakuweho ni 200μL, naho icyifuzo cyo gukuraho ni 80μL.
