Virusi ya dengue, virusi na vikurus na chikurunya virusi nyinshix
Izina ry'ibicuruzwa
Hwts-Fe040 Dengue virusi, virusi ya Zika na Chikurunya virusi ya Aciclex Nucleic yandika acide ya acidex. Fluorescence PCR)
Epidemiology
Umuriro wa Dengue (DF), watewe na virusi ya Dengue (Denv), ni kimwe mu birezo bya Arbovitic byinshi. Uburyo bwayo bwoherejwe burimo Aedes Aegndu na Adede Albopictus. DF yiganje cyane mubushyuhe nubusumbayi. Drevivirus uri munsi ya Flaviviridae, kandi irashobora gushyirwa mu mwanya wa 4 serotypes ukurikije ubuso bwa Antigen. Kugaragaza amavuriro ya Denv harimo umutwe, umuriro, intege nke, kwaguka kwa lymph node, LeukoPenia na ETC, guhungabana, gukomeretsa kwa sapatike cyangwa urupfu rwamabato. Mu myaka yashize, imihindagurikire y'ikirere, imijyi, iterambere ryihuse ry'ubukerarugendo n'izindi mpamvu zatanze umusaruro wihuse kandi woroshye wo kwanduza no gukwirakwiza DF, biganisha ku kwagura iki cyorezo cya DF.
Umuyoboro
| Umuryango | Acide nucleic nucleic |
| Rox | Kugenzura imbere |
Tekinike
| Ububiko | -18 ℃ |
| Imibereho-Ubuzima | Amezi 9 |
| Ubwoko bw'ikigereranyo | Serum nshya |
| Ct | ≤38 |
| CV | <5% |
| Lod | Kopi 500 / ML |
| Umwihariko | Ibisubizo by'ibizamini byo kwivanga byerekana ko iyo kwibanda kuri Bilirubin muri Serumu bitarenze 168. Kwibanda muri Serumu ntabwo birenze 5mg / ml, nta ngaruka kuri virusi ya dengue, virusi ya Zika cyangwa kwandura virusi ya vikungunya. Hepatite Arus, Virusi ya Hepatite B, virusi ya hepatite c virusi, virusi ya Herpefiya, Virusi ya Bunya, Ububiko bwa Nntavirus, Ububiko bwa NINYA, Ibisubizo byerekana Kwambukiranya hagati yibi bikoresho hamwe nimbaraga zavuzwe haruguru. |
| Ibikoresho bisabwa | Gukoresha Biosystems 7500 Igihe cya PCR Gushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Umutobe®5-igihe nyacyo PCR sisitemu Umucyo®480 nyamara-sisitemu ya PCR Linegene 9600 wongeyeho sisitemu yigihe cya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Ihitamo rya 1.
Vianamp Virus ADN / RNA Kit (YDP315-R), kandi gukuramo bigomba gukorwa mubuyobozi bukomeye bwo gukoresha. Igitabo cyitegererezo cyakuweho ni 140μl, kandi umubumbe wabisabwa ni 60μl.
IHitamo 2.
Macro & Micro-Ikizamini Rusange ADN / RNA Kit (HNAT-3017-50, hwts-3017-42, HWTS-3017-48, Ikizamini cya Acid Automatic Automatic Automatic (HWTS-3006C, HWTS-3006B)) by JingGu Macro & Micro-Ikizamini Med-Tech Com-Tech Co Ltd., kandi gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha. Igitabo cyitegererezo cyakuweho ni 200μl, hamwe nubunini bwabigenewe ni 80μl.
