Virusi ya Dengue, Virusi ya Zika na Virusi ya Chikungunya Multiplex
Izina ry'igicuruzwa
HWTS-FE040 Virusi ya Dengue, Virusi ya Zika na Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Ubumenyi bw'indwara
Indwara ya Dengue (DF), iterwa n'ubwandu bwa virusi ya Dengue (DENV), ni imwe mu ndwara zandura za arbovirus zikunze kugaragara cyane. Uburyo yanduriramo burimo Aedes aegypti na Aedes albopictus. Indwara ya Dengue ikunze kugaragara cyane mu turere dushyuha n'utwo mu turere dushyuha. Indwara ya Dengue ikomoka kuri flavivirus iri mu bwoko bwa flaviviridae, kandi ishobora gushyirwa mu byiciro 4 hakurikijwe antigen yo hejuru. Ibimenyetso by'ubwandu bwa DENV birimo kuribwa umutwe, umuriro, intege nke, kwiyongera kwa lymph node, leukopenia n'ibindi, no kuva amaraso, guhungabana, gukomereka mu mwijima cyangwa ndetse no gupfa mu bihe bikomeye. Mu myaka ya vuba aha, imihindagurikire y'ikirere, iterambere ryihuse ry'imijyi, iterambere ryihuse ry'ubukerarugendo n'ibindi bintu byatanze uburyo bwihuse kandi bworoshye bwo korohereza no gukwirakwiza DF, bigatuma agace k'icyorezo cya DF karushaho kwaguka.
Umuyoboro
| FAM | aside nucleike ya DENV |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | -18℃ |
| Igihe cyo kumara | Amezi 9 |
| Ubwoko bw'ingero | Serumu nshya |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | Kopi 500/mL |
| Ubwihariye | Ibisubizo by'ibizamini by'ingaruka z'amaraso bigaragaza ko iyo ubwinshi bwa bilirubin mu maraso butarenze 168.2μmol/ml, ubwinshi bwa hemoglobine bukorwa na hemolysis butarenze 130g/L, ubwinshi bwa lipide mu maraso butarenze 65mmol/ml, ubwinshi bwa IgG mu maraso butarenze 5mg/mL, nta ngaruka igira ku cyorezo cya dengue, virusi ya Zika cyangwa virusi ya chikungunya. Virusi ya Hepatite A, virusi ya Hepatite B, virusi ya Hepatite C, virusi ya Herpes, virusi ya Eastern equine encephalitis, virusi ya Hantavirusi, virusi ya Bunya, virusi ya West Nile na serum y'abantu byatoranijwe kugira ngo hakoreshwe ikizamini cya cross-reactivity, kandi ibisubizo bigaragaza ko nta mpungenge hagati y'iki gikoresho n'udukoko twavuzwe haruguru. |
| Ibikoresho Bikoreshwa | Sisitemu ya PCR ikoreshwa mu gihe nyacyo ya 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Amahitamo ya 1.
TIANamp Virus DNA/RNA Kit (YDP315-R), kandi gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha. Ingano y'icyitegererezo cyakuwemo ni 140μL, naho ingano ya elution isabwa ni 60μL.
Amahitamo ya 2.
Kit ya Macro & Micro-Test General ADN/RNA (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ya Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., kandi gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha. Ingano y'icyitegererezo cyakuwemo ni 200μL, naho ingano ya elution isabwa ni 80μL.
