Virusi ya Dengue I/II/III/IV aside nucleic
Izina ry'igicuruzwa
Kiti yo Gupima Aside ya Nucleic ya HWTS-FE034-Dengue Virus I/II/III/IV (Fluorescence PCR)
HWTS-FE004-Icyikoresho cyo Gupima Aside ya Nucleic ya HWTS-FE004 (Fluorescence PCR)
Icyemezo
CE
Ubumenyi bw'indwara
Indwara ya Dengue (DF), iterwa n'ubwandu bwa Denguevirus (DENV), ni imwe mu ndwara zandura za arbovirus zikunze kugaragara cyane. DENV ni iya flavivirus iri munsi ya flaviviridae, kandi ishobora gushyirwa mu byiciro bine by’ubwoko bwa serotypes hakurikijwe antigen yo hejuru. Uburyo bwo kuyikwirakwiza burimo Aedes aegypti na Aedes albopictus, ikunze kugaragara cyane mu turere dushyuha n’utwo mu turere dushyuha.
Ibimenyetso by’ubwandu bwa DENV birimo cyane cyane kuribwa umutwe, umuriro, gucika intege, kwiyongera kwa limfu, leukopenia n'ibindi, kuva amaraso, gutungurwa, gukomereka mu mwijima cyangwa ndetse no gupfa mu bihe bikomeye. Mu myaka ya vuba aha, imihindagurikire y'ikirere, iterambere ryihuse ry'ubukerarugendo n'ibindi bintu byatanze uburyo bwihuse kandi bworoshye bwo kwanduza no gukwirakwiza DF, bigatuma agace k'icyorezo cya DF karushaho kwaguka.
Umuyoboro
| FAM | Virusi ya Dengue I |
| VIC (HEX) | Virusi ya Dengue II |
| ROX | Virusi ya Dengue III |
| CY5 | Virusi ya Dengue IV |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18℃ mu mwijima; lyophilization: ≤30℃ mu mwijima |
| Igihe cyo kumara | Amazi: amezi 9; lyophilization: amezi 12 |
| Ubwoko bw'ingero | Serumu nshya |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | Kopi 500/mL |
| Ubwihariye | Kora ibizamini bya virusi ya encephalitis yo mu Buyapani, virusi ya encephalitis yo mu ishyamba, umuriro ukomeye ufite thrombocytopenia syndrome, umuriro wo kuva amaraso muri Xinjiang, virusi ya hantaan, virusi ya hepatite C, virusi ya grippe A, virusi ya grippe B n'ibindi. Nta bimenyekanye bya cross reaction. |
| Ibikoresho Bikoreshwa | Ishobora guhuza n'ibikoresho bya PCR bikoreshwa mu gupima urumuri biri ku isoko. Sisitemu za PCR za SLAN-96P mu gihe nyacyo Sisitemu za PCR za ABI 7500 mu gihe nyacyo Sisitemu za PCR za ABI 7500 zihuta cyane mu gihe nyacyo Sisitemu za PCR za QuantStudio®5 mu gihe nyacyo Sisitemu za PCR za LightCycler®480 mu gihe nyacyo Sisitemu zo Gusuzuma za LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Igisubizo cya PCR cyose
