Carbapenem Kurwanya Gene (KPC / NDM / OXA 48 / OXA 23 / VIM / IMP)
Izina RY'IGICURUZWA
HWTS-OT045 Carbapenem Kurwanya Gene (KPC / NDM / OXA 48 / OXA 23 / VIM / IMP) Igikoresho cyo kumenya (Fluorescence PCR)
Epidemiologiya
Antibiyotike ya Carbapenem ntisanzwe ant-antibiyotike ya lactam hamwe na antibacterial nini yagutse hamwe nibikorwa bikomeye bya antibacterial.Kubera ituze kuri β-lactamase n'uburozi buke, yabaye imwe mu miti ikomeye ya antibacterial yo kuvura indwara ziterwa na bagiteri.Carbapenem irahagaze neza cyane kuri plasmid-yunganirwa yagutse-ya-lactamase (ESBLs), chromosomes, hamwe na cephalosporinase ya plasmid-yunganirwa (enzymes ya AmpC).
Umuyoboro
| PCR-Kuvanga 1 | PCR-Kuvanga 2 | |
| FAM | IMP | VIM |
| VIC / HEX | Igenzura ryimbere | Igenzura ryimbere |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | Amacandwe, koloni nziza, swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU / mL |
| Umwihariko | a) Igikoresho cyerekana isosiyete isanzwe yemewe, kandi ibisubizo byujuje ibisabwa byerekeranye. b) Ibyavuye mu kizamini cya cross-reactivite byerekana ko iki gikoresho kidafite aho gihurira nizindi ndwara zandurira mu myanya y'ubuhumekero, nka Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus oxyde, Kle, junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, respirator adenovirus, Enterococcus, cyangwa ingero zirimo izindi gen zirwanya ibiyobyabwenge CTX, mecA, SME, SHV, TEM, nibindi c) Kurwanya-kwivanga: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, acide Clavulanic, Roxithromycine byatoranijwe kugira ngo bipimishe, kandi ibisubizo byerekana ko ibintu bitavanze byavuzwe haruguru. kugirango hamenyekane genes zo kurwanya karbapenem KPC, NDM, OXA48, OXA23, VIM, na IMP. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR SLAN-96P Sisitemu nyayo-PCR (Hongshi Medical Technology Co., Ltd.) UmucyoCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR ()FQD-96A，HangzhouIkoranabuhanga rya Bioer) MA-6000 Igihe Cyuzuye Cyumubare Wumukino Wamagare (Suzhou Molarray Co., Ltd.) BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Ihitamo 1.
Gusabwa gukuramo reagent: Macro & Micro-Ikizamini rusange ADN / RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96). imvura igwa.Intambwe zikurikira zigomba gukurikiza amabwiriza yo gukuramo, kandi ingano yo gusabwa ni100μL.
Icya 2.
Gusabwa gukuramo reagent: Gukuramo Acide Nucleic Acide cyangwa Isukura Reagent (YDP302) na Tiangen Biotech (Beijing) Co., Ltd. , no kunyeganyega kugeza thallus ihagaritswe burundu).Koresha amazi yubusa ya RNase / DNase kugirango ukureho, kandi ingano ya elution yatanzwe ni 100μL.
Icya 3.
Basabwe gukuramo reagent: Macro & Micro-Ikigereranyo Cyitegererezo cyo Kurekura Reagent.Urugero rwa spumum rugomba gukaraba wongeyeho 1mL yumunyu usanzwe mumvura yavuzwe haruguru ya thallus yavuzwe haruguru, igashyirwa kuri 13000r / min muminota 5, hanyuma ndengakamere ikajugunywa (komeza 10-20µL yindengakamere).Kuri koloni isukuye hamwe na swab ya rectal, ongeramo 50μL yicyitegererezo cyo kurekura reagent kumurongo wavuzwe haruguru wavuwe na thallus, hanyuma intambwe ikurikiraho igomba gukururwa ukurikije amabwiriza yo gukoresha.
