Generapenem Kurwanya Gene (KPC / NDM / OXA 48 / OXA 23 / VIM / Imp)
Izina ry'ibicuruzwa
Hwts-ot045 Carbapenem Kurwanya Gene (KPC / NDM / Oxa 48 / Oxa 23 / VIM / Imp) Gutahura Kit (Fluorescence PCR)
Epidemiology
Carbapenem antibionem ni atypical β-lacatm antibiyotike hamwe na antibacterial spectrum hamwe nibikorwa bikomeye bya antibacteri. Kubera ituze ryayo kuri β - kubura uburozi buke, byabaye imwe mu miti ikomeye ya antibacterie yo kuvura indwara za bagiteri zikomeye. Carbapenez ihamye cyane kuri plasmid yaguye-spectrum β-lactrum (esbls), chromosomes, na plasmid-bisukuye kuri clasimes (ampc enzymes).
Umuyoboro
| PCR-Kuvanga 1 | PCR-Kuvanga 2 | |
| Umuryango | Imp | Vim |
| Vic / Hex | Kugenzura imbere | Kugenzura imbere |
| Cy5 | NDM | Kpc |
| Rox | Oxa48
| Oxa23 |
Tekinike
| Ububiko | ≤-18 ℃ |
| Imibereho-Ubuzima | Amezi 12 |
| Ubwoko bw'ikigereranyo | Sputum, ubukoloni bwera, swil swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| Lod | 103CFU / ML |
| Umwihariko | A) Ibikoresho bitahura isosiyete isanzwe yerekanwe, kandi ibisubizo byujuje ibyangombwa bisabwa. b) Ibisubizo by'ibizamini byambukiranya abantu byerekana ko iyi Kit idafite reaction yambukiranya, Acidenacter Umuryango wa Acinanniae, Spebtoonacter Pineniya, KlebyLella Oxytoca, Haemophilus Driveenzae, Acinetbacter Junii, Acinetla Smo, Shv, Tem, nibindi c) Kurwanya: Mucin, Minocycline, Candamicin, Clindamycin, Imipiraméméecide, kandi ibisubizo byerekana ko ibintu byavuzwe haruguru bivuga haruguru bidafite reaction Kumenya Karbapenem yo kurwanya genes zen, NDM, Oxa48, Oxa23, VIM, na Imp. |
| Ibikoresho bisabwa | Gushyira mu bikorwa biosystems 7500 igihe cya sisitemu ya PCR Quartudio®5 sisitemu nyayo PCR Slan-96p Sisitemu NYAKURI PCR (Tekinoroji yubuvuzi Cology Co, Ltd.) Umucyo®480 Igihe Cyigihe cya PCR Linegene 9600 wongeyeho sisitemu yigihe cya PCR (FQD-96A,HangzhouIkoranabuhanga rya Bioer) Ma-6000 nyayo-nyayo Umwanya wa Cyrmal Cycler (Suzhou Morlary Co, Ltd.) Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Ihitamo rya 1.
Basabwe gukuramo Reagent: Macro & Micro-Ikizamini Rusange ADN / RNA Kit (HWTS-3019-50, hwts-3019-32, hwts-3019-48, hwts-3019-96) Thallus. Intambwe yakurikiyeho igomba gukurikiza amabwiriza yo gukuramo, kandi ingano yasabwe ni100μl.
IHitamo 2.
Gusaba gukuramo Reagent: Gukuramo acide nucleic cyangwa kweza , no kunyeganyega kugeza thallus yahagaritswe rwose). Koresha RNASE / DNsenge amazi yubusa yo kwishyurwa, kandi ingano yo hejuru ya ellsure ni 100μl.
Ihitamo rya 3.
Gusaba gukuramo Reagent: Macro & Micro-Ikizamini Icyitegererezo cya Redle Kurekura reagent. Icyitegererezo cya Sputom kigomba gukaraba no kongeramo 1ml yiminyu yo mu minyururu isanzwe yavuzwe haruguru, Centrifuged saa kumi n'ebyiri zavuzwe haruguru, yajugunywe mu minota 5, kandi umugozi wa 10-20μl wa ndenga). Kubijyanye n'ubukoloni bwiza na nyakakira, ongeramo 50μl of Samle Kurekura reagent bitaziguye bitavuzwe haruguru imvura yavuzwe haruguru, kandi intambwe nyuma igomba gukurwa ukurikije amabwiriza yo gukoresha.
