ALDH Polymorphism y'ibimera
Izina ry'igicuruzwa
HWTS-GE015Kit yo Gusuzuma Ubumuga bwa ALDH (ARMS -PCR)
Ubumenyi bw'indwara
Gene ya ALDH2 (acetaldehyde dehydrogenase 2), iherereye kuri chromosome y'umuntu 12. ALDH2 ifite esterase, dehydrogenase na reductase activity icyarimwe. Ubushakashatsi bwagaragaje ko ALDH2 ari enzyme ya metabolism ya nitroglycerin, ihindura nitroglycerin mo nitric oxide, bityo ikaruhura imiyoboro y'amaraso kandi ikanoza imiyoboro y'amaraso. Ariko, hari polymorphisms muri gene ya ALDH2, ikunze kuba muri Aziya y'Iburasirazuba. ALDH2*1/*1 GG yo mu bwoko bwa wild-type ifite ubushobozi bukomeye bwo metabolism, mu gihe ubwoko bwa heterozygous bufite 6% gusa by'imikorere ya enzyme yo mu bwoko bwa wild-type, naho ubwoko bwa homozygous mutant bufite hafi ya zeru ya enzyme, hamwe na metabolisme iba nke cyane kandi ntishobora kugera ku ngaruka zifuzwa, bityo bigatera ingaruka mbi ku mubiri w'umuntu.
Umuyoboro
| FAM | ALDH2 |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Amaraso adafite amaraso menshi ya EDTA |
| CV | <5.0% |
| LoD | 103Kopi/mL |
| Ibikoresho Bikoreshwa | Sisitemu ya PCR ikoreshwa mu gihe nyacyo ya 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Ibikoresho byo gukuramo ADN by’amaraso byasabwe: koresha Blood Genome DNA Extraction Kit (DP318) ya Tiangen Biotech (Beijing) Co., Ltd. cyangwa Blood Genome Extraction Kit (A1120) ya Promega kugira ngo ukure ADN ya Genome y’amaraso idafite amaraso menshi.
Ibikoresho byo gukuramo ibinyabutabire byasabwe: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Gukuramo ibinyabutabire bigomba gukorwa hakurikijwe amabwiriza neza. Ingano ya elution isabwa ni100μL.
