Adenovirus Yisi Yose
Izina RY'IGICURUZWA
HWTS-RT017A Adenovirus Universal Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Adenovirus ya muntu (HAdV) ni iy'ubwoko bwa Mammalian adenovirus, ikaba ari virusi ya ADN ikubye kabiri idafite ibahasha.Adenovirusi zabonetse kugeza ubu zirimo amatsinda 7 mato (AG) n'ubwoko 67, muri zo serotipi 55 zitera abantu.Muri byo, bishobora gutera indwara zandurira mu myanya y'ubuhumekero ahanini ni itsinda B (Ubwoko 3, 7, 11, 14, 16, 21, 50, 55), Itsinda C (Ubwoko 1, 2, 5, 6, 57) n'itsinda E (Ubwoko 4), kandi bishobora gutera kwandura munda ni amatsinda F (Ubwoko 40 na 41) [1-8].Ubwoko butandukanye bufite ibimenyetso byubuvuzi bitandukanye, ariko cyane cyane indwara zubuhumekero.Indwara z'ubuhumekero ziterwa n'indwara z'ubuhumekero zanduza umubiri w'umuntu zifite 5% ~ 15% by'indwara z'ubuhumekero ku isi, na 5% -7% by'indwara z'ubuhumekero ku isi [9].Adenovirus yanduye ahantu henshi kandi irashobora kwandura umwaka wose, cyane cyane ahantu huzuye abantu, bakunze kwibasirwa n’aho, cyane cyane mu mashuri no mu nkambi za gisirikare.
Umuyoboro
| FAM | adenovirus kwisi yoseaside nucleic |
| ROX | Igenzura ryimbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | Nasopharyngeal swab，Umuhogo |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300Kopi / mL |
| Umwihariko | a) Gerageza isosiyete isanzwe ikoreshwa nabi nibikoresho, kandi ibisubizo byikizamini byujuje ibisabwa. b) Koresha iki gikoresho kugirango umenye kandi ntihabeho kwisubiraho hamwe nizindi ndwara ziterwa nubuhumekero (nka virusi ya grippe A, virusi ya grippe B, virusi yubuhumekero, virusi ya Parainfluenza, Rhinovirus, metapneumovirus, nibindi) cyangwa bagiteri (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, nibindi). |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu-Igihe nyacyo PCR SLAN-96P Sisitemu nyayo-PCR (Hongshi Medical Technology Co., Ltd.) UmucyoCycler®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCRs (FQD-96A, HangzhouIkoranabuhanga rya Bioer) MA-6000 Igihe Cyuzuye Cyumubare Wumukino Wamagare (Suzhou Molarray Co., Ltd.) BioRad CFX96 Sisitemu Yigihe-PCR Sisitemu, BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
(1) Basabwe gukuramo reagent:Macro & Micro-Ikigereranyo Icyitegererezo cyo Kurekura Reagent (HWTS-3005-8).Gukuramo bigomba gukorwa ukurikije amabwiriza.Icyitegererezo cyakuweho ni abarwayi'nasopharyngeal swab cyangwa umuhogo swab ingero zegeranijwe kurubuga.Ongeraho icyitegererezo muri sample yo gusohora reagent na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex kuvanga neza, shyira mubushyuhe bwicyumba muminota 5, fata hanyuma uhindure hanyuma uvange neza kugirango ubone ADN ya buri cyitegererezo.
(2) Basabwe gukuramo reagent:Macro & Micro-Ikizamini Virus ADN / RNA Kit(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Ikizamini cyikora Nucleic Acide ikuramo (HWTS-3006C, HWTS-3006B).igikorwa kigomba gukorwa hakurikijwe amabwiriza.Ingano yicyitegererezo yakuweho ni 200μL, nabyasabwe kuvunikais80μL.
.315) na Tiangen Biotech (Beijing) Co, Ltd.., iibikorwa bigomba gukorwa bikurikije amabwiriza.Ingano yicyitegererezo yakuweho ni 200μL, nabyasabwe kuvunikais80μL.
