Adenovirus Universal
Izina ry'igicuruzwa
HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Ubumenyi bw'indwara
Virusi ya adenovirusi y’abantu (HAdV) iri mu bwoko bwa adenovirusi ya Mammalian, ikaba ari virusi ya ADN ifite imigozi ibiri idafite aho ibogamiye. Virusi ya adenovirusi yabonetse kugeza ubu irimo amatsinda 7 (AG) n’ubwoko 67, muri yo 55 serotypes ikaba itera indwara ku bantu. Muri zo, zishobora gutera indwara zo mu myanya y’ubuhumekero ni itsinda rya B (Ubwoko bwa 3, 7, 11, 14, 16, 21, 50, 55), Itsinda rya C (Ubwoko bwa 1, 2, 5, 6, 57) n’Itsinda rya E (Ubwoko bwa 4), kandi zishobora gutera indwara yo gucibwamo mu mara ni Itsinda rya F (Ubwoko bwa 40 na 41) [1-8]. Ubwoko butandukanye bufite ibimenyetso bitandukanye by’indwara, ariko cyane cyane indwara zo mu myanya y’ubuhumekero. Indwara zo mu myanya y’ubuhumekero ziterwa n’indwara zo mu myanya y’ubuhumekero mu mubiri w’umuntu zigize 5% ~ 15% by’indwara zo mu myanya y’ubuhumekero ku isi, na 5% - 7% by’indwara zo mu myanya y’ubuhumekero ku isi mu bana [9]. Indwara ya Adenovirusi ikunze kugaragara mu duce twinshi kandi ishobora kwandura umwaka wose, cyane cyane mu duce dutuwe n’abantu benshi, dukunze kwibasirwa n’ibyorezo byo mu gace runaka, cyane cyane mu mashuri no mu nkambi za gisirikare.
Umuyoboro
| FAM | virusi ya adenovirusi rusangeaside nucleique |
| ROX | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Agatambaro ko mu mazuru，Agapfukamunwa ko mu muhogo |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Kopi 300/mL |
| Ubwihariye | a) Gerageza ibimenyetso byagenwe by’uko ikigo gitanga serivisi mbi, kandi ibisubizo by’ikizamini byujuje ibisabwa. b) Koresha iyi mashini kugira ngo umenye neza ko nta ndwara ishobora gutera mu buryo butandukanye n’izindi ndwara zitera indwara zo mu myanya y’ubuhumekero (nk’indwara ya Grippe A, virusi ya Grippe B, virusi ya Respiratory syncytial virus, Parainfluenza virus, Rhinovirus, Human metapneumovirus, n’izindi) cyangwa bagiteri (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, n’izindi). |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 Sisitemu za PCR za QuantStudio®5 mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.) Sisitemu ya LightCycler®480 Real-Time PCR Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyos (FQD-96AHangzhouIkoranabuhanga rya Bioer) MA-6000 Real-Time Quantitative Thermocycler (Suzhou Molarray Co., Ltd.) Sisitemu za BioRad CFX96 za PCR mu gihe nyacyo, Sisitemu za BioRad CFX Opus 96 za PCR mu gihe nyacyo |
Urugendo rw'akazi
(1) Ikoreshwa ry’ibinyabutabire risabwa gukurwamo:Ikizamini cya Macro na Micro-Test Release Reagent (HWTS-3005-8). Gukuramo bigomba gukorwa hakurikijwe amabwiriza. Ikizamini cyakuwemo ni abarwayi'ingero z'agahu ka nasopharyngeal cyangwa agahu ka mu muhogo byakusanyijwe aho biri. Shyiramo ingero mu gikoresho cyo kurekura ingero cyakozwe na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex kugira ngo bivange neza, bishyire ku bushyuhe bw'icyumba mu gihe cy'iminota 5, ubikuremo hanyuma ubihindure uvange neza kugira ngo ubone ADN ya buri gikoresho.
(2) Uburyo bwo gukuramo ibintu busabwa:Isuzuma rya Macro na Micro Virusi Kit ya ADN/RNA(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Igikorwa kigomba gukorwa hakurikijwe amabwiriza neza. Ingano y'icyitegererezo cyakuwemo ni 200μL, kandiingano y'umuti usabwais80μL.
(3) Reagent isabwa gukurwamo: Ikoreshwa rya aside nucleic cyangwa Reagent yo gusukura (YDP)315) na Tiangen Biotech (Beijing) Co., Ltd.,Igikorwa kigomba gukorwa hakurikijwe amabwiriza neza. Ingano y'icyitegererezo cyakuwemo ni 200μL, kandiingano y'umuti usabwais80μL.
