Ubwoko 28 bwa virusi ya Papillomavirusi y'umuntu (16/18/45Typing) Kit yo Gupima Acide Nucleic (Fluorescence PCR)
Izina ry'igicuruzwa
Ubwoko 28 bwa virusi ya Papillomavirusi y'umuntu (16/18/45Typing) Kit yo Gupima Acide Nucleic (Fluorescence PCR)
Ubumenyi bw'indwara
Virusi ya Human Papillomavirus (HPV) iri mu muryango wa Papillomaviridae w’agakoko gato ka ADN kadafite ipfundo, gafite imigozi ibiri, gafite uburebure bwa genome bugera kuri pairs 8000 (bp). HPV yandura abantu binyuze mu gukoranaho ibintu byanduye cyangwa byandujwe mu mibonano mpuzabitsina. Iyi virusi ntabwo ari iy’umuntu gusa, ahubwo inakwirakwira mu mubiri, kandi ishobora kwanduza gusa uturemangingo tw’uruhu rw’umuntu n’uturemangingo tw’imitsi, bigatera ubwoko butandukanye bwa papilloma cyangwa ibiheri mu ruhu rw’umuntu ndetse no kwangiza epithelium y’imyororokere.
Kanseri y'inkondo y'umura ni imwe mu ndwara zikunze kugaragara mu myanya myibarukiro y'abagore. Ubushakashatsi bwagaragaje ko kwandura HPV guhoraho n'indwara nyinshi ari imwe mu mpamvu zikomeye zitera kanseri y'inkondo y'umura. Kuri ubu, uburyo buzwi bwo kuvura kanseri y'inkondo y'umura buracyari buke, bityo kuvumbura no gukumira ubwandu bw'inkondo y'umura buterwa na HPV ni ingenzi mu gukumira kanseri y'inkondo y'umura. Ni ingenzi cyane gushyiraho ikizamini cyoroshye, cyihariye kandi cyihuse cyo gusuzuma indwara kugira ngo hamenyekane kandi havurwe kanseri y'inkondo y'umura.
Ibipimo bya tekiniki
| Ububiko | ≤ -18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Uumugatiicyitegererezo,FIcyitegererezo cy'umuti wo mu maraso (emale cervical swab), FIcyitegererezo cy'isukari yo mu gitsina cy'umuntu (emale) |
| CV | <5.0% |
| LoD | Umupaka wo Kumenya (LoD): LoD y'ibikoresho ni 300Copies/mL |
| Ubwihariye | Nta gusubiza hamwehamwe n'udukoko dutera indwara zisa. |
| Ibikoresho Bikoreshwa | Sisitemu ya PCR ikoreshwa mu buryo bw'umwimerere 7500, Sisitemu ya PCR ikoreshwa mu buryo bw'ihuse 7500, Sisitemu ya PCR ikoreshwa mu buryo bw'umwimerere ya QuantStudio®5, Sisitemu ya PCR ikoreshwa mu buryo bw'umwimerere ya SLAN-96P (Hongshi Medical Technology Co., Ltd.), Sisitemu ya PCR ikoreshwa mu buryo bw'umwimerere ya LightCycler®480, Sisitemu zo Gupima PCR zikoreshwa mu buryo bw'umwimerere za LineGene 9600 Plus (FQD-96A,HangzhouIkoranabuhanga rya Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System na BioRad CFX Opus 96 Real-Time PCR System.
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Umuyoboro:
| Impuguke mu gusubiza | Fluorophore | Umuzimyamwoto |
| PCR-Mix1/PCR-Mix2 | FAM | nta na kimwe |
| VIC(HEX) | nta na kimwe | |
| ROX | nta na kimwe | |
| CY5 | nta na kimwe |
Imikorere y'akazi: Reba Kit IFU kugirango umenye ibisobanuro birambuye
Igisubizo cya 1 (Gukuramo aside nucleique mu buryo bwikora)
Igisubizo cya 2: (Icyitegererezo cyo gusohora icyitegererezo cya Macro na Micro-Test)
Ibikoresho bitanga umusaruro bigomba ariko ntibitangwa:Ikizamini cya Macro & Micro-Test Release Reagent (HWTS-3005-8), Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).
Ibikoresho bikoreshwa bigomba ariko ntibitangwa: Nta DNase/RNase ifite 1.5mLcibinyabutabiretubube,DIzuru/RAmanota adafite Nase, 0.2mL y'imiyoboro ya PCR, centrifuge yo kuri desktop, imvange yo gusimbuka kuri desktop.
