Ubwoko 28 bwa Virusi ya Papilloma y'Umuntu (Type 16/18) ya aside nucleic
Izina ry'igicuruzwa
HWTS-CC006A-28 Ubwoko bwa Virusi ya Papilloma y'Umuntu ifite ibyago byinshi (Typing 16/18) Kit yo Gupima Acide Nucleic (Fluorescence PCR)
Ubumenyi bw'indwara
Kanseri y'inkondo y'umura ni imwe mu ndwara zikunze kugaragara mu myanya myibarukiro y'abagore. Ubushakashatsi bwagaragaje ko kwandura HPV guhoraho n'indwara nyinshi ari imwe mu mpamvu zikomeye zitera kanseri y'inkondo y'umura. Kuri ubu, uburyo buzwi bwo kuvura kanseri y'inkondo y'umura buracyari buke, bityo kuvumbura no gukumira ubwandu bw'inkondo y'umura buterwa na HPV ni ingenzi mu gukumira kanseri y'inkondo y'umura. Ni ingenzi cyane gushyiraho ikizamini cyoroshye, cyihariye kandi cyihuse cyo gusuzuma indwara kugira ngo hamenyekane kandi havurwe kanseri y'inkondo y'umura.
Umuyoboro
| Uruvange rw'ibisubizo | Umuyoboro | Ubwoko |
| PCR-Mix1 | FAM | 18 |
| VIC (HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Igenzura ry'imbere | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC (HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Igitambaro cyo mu nda, Igitambaro cyo mu gitsina, Inkari |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | Kopi 300/mL |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo LightCycler®Sisitemu ya PCR ya 480 mu gihe nyacyo Sisitemu yo Gusuzuma PCR ya LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Reagent isabwa gukurwamo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ongeraho 200μL y'umunyu usanzwe kugira ngo wongere ushyiremo agace gato mu ntambwe ya 2.1, hanyuma gukurwamo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha iyi reagent isabwa gukurwamo. Ingano ya elution isabwa ni 80μL.
Reagent yo gukuramo isabwa: QIAamp DNA Mini Kit (51304) cyangwa Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Ongeraho 200μL y'umunyu usanzwe kugira ngo wongere ushyiremo agace gato mu ntambwe ya 2.1, hanyuma gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha iyi reagent yo gukuramo. Ingano y'icyitegererezo cyakuwemo ni 200μL, kandi ingano y'icyitegererezo cyatanzwe ni 100μL.
