Ubwoko 28 bwintangarugero-cyane vipilloma yumuntu wa papilloma (16/18 Kwandika) acide ya nucleic
Izina ry'ibicuruzwa
HWTS-CCAP6A-28 Ubwoko bw'ibyago Byinshi PAPIlloma
Epidemiology
Kanseri y'inkondo y'umura nimwe mubyifuzo bikunze kugaragara mubitabo byimyororokere yumugore. Ubushakashatsi bwerekanye ko HPV ihora indwara kandi indwara nyinshi ni imwe mu mpamvu nyamukuru zitera kanseri y'inkondo y'umura. Kugeza ubu, uburyo bwemewe bwo kuvura buracyabura kanseri y'inkondo y'umura yatewe na HPV, mu buryo bwavuzwe hakiri kare no gukumira indwara y'inkondo y'umura yatewe na HPV urufunguzo rwo gukumira kanseri y'inkondo y'umura. Ni ngombwa gushinga ibizamini byoroshye, byihariye kandi byihuse kubizamini byubuvuzi no kuvura kanseri y'inkondo y'umura.
Umuyoboro
| Ivanga | Umuyoboro | Ubwoko |
| PCR-Mix1 | Umuryango | 18 |
| Vic (hex) | 16 | |
| Rox | 31, 35, 39, 51, 52, 56, 58, 58, 59, 66, 68 | |
| Cy5 | Kugenzura imbere | |
| PCR-Mix2 | Umuryango | 6, 11, 54, 83 |
| Vic (hex) | 26, 44, 61, 81 | |
| Rox | 40, 42, 43, 53, 73, 82 | |
| Cy5 | Kugenzura imbere |
Tekinike
| Ububiko | Amazi: ≤-18 ℃ |
| Imibereho-Ubuzima | Amezi 12 |
| Ubwoko bw'ikigereranyo | Swab y'inkondo y'umura, uruvange rwa swab, inkari |
| Ct | ≤28 |
| CV | <5.0% |
| Lod | 300copies / ml |
| Ibikoresho bisabwa | Gushyira mu bikorwa biosystems 7500 igihe cya sisitemu ya PCR Gushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Umutobe®5-igihe nyacyo PCR sisitemu Scan-96p sisitemu nyayo pcr sisitemu Umucyo®480 nyamara-sisitemu ya PCR Linegene 9600 wongeyeho sisitemu yigihe cya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Gusaba Gukuramo Reagent: Macro-Ikizamini cya Viral ADN / RNA Kit (Hnats-3017-50, hwts-3017-48, HWTS-3017-96) (Birashobora gukoreshwa hamwe na macro & micro-ikizamini Automatic Autocctor ya acide (HWTS-3006C, HWTS-3006B)) na Jiagsu Macro & Micro-Ikizamini Med-Tech Co., Ltd. Ongeraho 200μl yumunyu wa saline mu buryo busanzwe bwo kwigumya pellet mu ntambwe mu ntambwe y'intambwe ya 2.1, hanyuma igura ikwiye gukorwa hakurikijwe amabwiriza yo gukoresha iyi reagen. Umubumbe w'ikirenga usabwa ni 80μl.
Gusaba gukuramo Reagent: Qiaimp DNna Mini Kit (51304) cyangwa Macro & micro-ikizamini cya virumbe ya virumbo ya virusi ya virusi ya virusi ya viruman (hwts (hwts-3020-50). Ongeramo 200μl yumunyu usanzwe wo kwigumya pellet muntambwe ya 2.1, hanyuma urwanywa zigomba gukorwa hakurikijwe amabwiriza yo gukoresha iki gikanda. Ingano yicyitegererezo yakuweho yingengo za 200μl, hamwe nubunini bwabigenewe ni 100μl.
