28 Ubwoko bwa virusi Yumuntu Papilloma (16/18 Kwandika) Acide Nucleic
Izina RY'IGICURUZWA
HWTS-CC006A-28 Ubwoko bwa virusi ya Papilloma Yumuntu Yanduye (16/18 Kwandika) Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Kanseri y'inkondo y'umura ni kimwe mu bibyimba bikunze kugaragara mu myanya myororokere y'abagore.Ubushakashatsi bwerekanye ko HPV yanduye kandi yanduye ni imwe mu mpamvu zitera kanseri y'inkondo y'umura.Kugeza ubu, imiti izwi neza iracyabura kanseri y'inkondo y'umura iterwa na HPV, bityo kuvumbura hakiri kare no kwirinda kwandura inkondo y'umura iterwa na HPV ni urufunguzo rwo kwirinda kanseri y'inkondo y'umura.Ni ingirakamaro cyane gushyiraho ikizamini cyoroshye cyo gusuzuma indwara ya etiologiya yo kwisuzumisha no kuvura kanseri y'inkondo y'umura.
Umuyoboro
| Kuvangavanga | Umuyoboro | Andika |
| PCR-Kuvanga1 | FAM | 18 |
| VIC (HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Igenzura ryimbere | |
| PCR-Kuvanga2 | FAM | 6, 11, 54, 83 |
| VIC (HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Igenzura ryimbere |
Ibipimo bya tekiniki
| Ububiko | Amazi: ≤-18 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | Inkondo y'umura Sw Vaginal Swab 、 Inkari |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300Kopi / mL |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho Sisitemu-Igihe Cyuzuye PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Gusabwa gukuramo reagent: Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa na Macro & Micro-Ikizamini Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ongeramo 200μL yumunyu usanzwe kugirango uhagarike pellet muntambwe 2.1, hanyuma gukuramo bigomba gukorwa ukurikije ku mabwiriza yo gukoresha iyi gukuramo reagent.Ingano isabwa yo gukuraho ni 80μL.
Gusabwa gukuramo reagent: QIAamp ADN Mini Kit (51304) cyangwa Macro & Micro-Test Virus ADN / Inkingi ya RNA (HWTS-3020-50).Ongeramo 200μL ya saline isanzwe kugirango uhagarike pellet muntambwe 2.1, hanyuma gukuramo bigomba gukorwa ukurikije amabwiriza yo gukoresha iyi reagent.Ingano yicyitegererezo yakuweho ni 200μL, kandi ingano yo gusabwa ni 100μL.
