Ubwoko bwa HPV 17 (18/6/6/11/44 Kwandika)
Izina RY'IGICURUZWA
HWTS-CC015 17 Ubwoko bwa Papillomavirus Yabantu (16/18/6/11/44 Kwandika) Kit Nucleic Acide Detection Kit (Fluorescence PCR)
Epidemiologiya
Kanseri y'inkondo y'umura ni kimwe mu bibyimba bikunze kugaragara mu myororokere y'abagore.Byerekanwe ko kwandura HPV no kwandura indwara nyinshi ari imwe mu mpamvu zitera kanseri y'inkondo y'umura.Kugeza ubu haracyari ikibazo cyo kuvura muri rusange kanseri ifata kanseri y'inkondo y'umura iterwa na HPV.Kubwibyo, gutahura hakiri kare no kwirinda kwandura inkondo y'umura iterwa na HPV ni urufunguzo rwo kwirinda kanseri y'inkondo y'umura.Gushiraho ibizamini byoroshye, byihariye kandi byihuse byo gusuzuma indwara ziterwa na virusi bifite akamaro kanini mugupima kanseri y'inkondo y'umura.
Umuyoboro
| PCR-Kuvanga1 | FAM | 18 |
| VIC / HEX | 16 | |
| ROX | 31，33，35，39，45，51，52，56，58，59，66，68 | |
| CY5 | Kugenzura imbere |
| PCR-Kuvanga2 | FAM | 6 |
| VIC / HEX | 11 | |
| ROX | 44 | |
| CY5 | Kugenzura imbere |
Ibipimo bya tekiniki
| Ububiko | -18 ℃ |
| Ubuzima bwa Shelf | Amezi 12 |
| Ubwoko bw'icyitegererezo | Icyitegererezo cy'inkari, icyitegererezo cy'umugore w'inkondo y'umura, icyitegererezo cy'igitsina gore |
| Ct | ≤28 |
| LoD | 300Kopi / mL |
| Umwihariko | Nta reaction-reaktivi hamwe na Ureaplasma urealyticum, Chlamydia trachomatis yinzira yimyororokere, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold, Gardnerella nubundi bwoko bwa HPV butapfukiranwa nibikoresho. |
| Ibikoresho bikoreshwa | Ikoreshwa rya Biosystems 7500 Sisitemu-Igihe nyacyo PCR Ikoreshwa rya Biosystems 7500 Byihuse-Igihe-Sisitemu ya PCR QuantStudio®5 Sisitemu nyayo-PCR SLAN-96P Sisitemu nyayo-PCR Umucyo®480 Sisitemu nyayo-PCR LineGene 9600 Yongeyeho-Igihe-Cyukuri cya PCR MA-6000-Igihe-Cyuzuye Cyumubare Wubushyuhe BioRad CFX96 Sisitemu-Igihe Cyuzuye PCR BioRad CFX Opus 96 Sisitemu Yigihe-PCR |
Urujya n'uruza rw'akazi
Gusabwa gukuramo reagent: Macro & Micro-Ikizamini cya virusi ADN / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa na Macro & Micro-Ikizamini Automatic Nucleic Acide Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ongeramo 200μL yumunyu usanzwe kugirango uhagarike pellet muntambwe 2.1, hanyuma gukuramo bigomba gukorwa ukurikije ku mabwiriza yo gukoresha iyi gukuramo reagent.Ingano isabwa yo gukuraho ni 80μL.
Gusabwa gukuramo reagent: QIAamp ADN Mini Kit (51304) cyangwa Macro & Micro-Test Virus ADN / Inkingi ya RNA (HWTS-3020-50).Ongeramo 200μL ya saline isanzwe kugirango uhagarike pellet muntambwe 2.1, hanyuma gukuramo bigomba gukorwa ukurikije amabwiriza yo gukoresha iyi reagent.Ingano yicyitegererezo yakuweho ni 200μL, kandi ingano yo gusabwa ni 100μL.
Gusabwa gukuramo reagent: Macro & Micro-Ikigereranyo Cyicyitegererezo cyo Kurekura Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L).Ongeramo 200μL yicyitegererezo cyo kurekura reagent kugirango uhagarike pellet muntambwe 2.1, hanyuma gukuramo bigomba gukorwa ukurikije amabwiriza yo gukoresha iyi reagent.
