Ubwoko 17 bwa HPV (16/18/6/11/44 Imyandikire)
Izina ry'igicuruzwa
HWTS-CC015 Ubwoko 17 bwa virusi ya Papillomavirusi y'abantu (16/18/6/11/44 Ubwoko) Agakoresho ko Gupima Aside Nucleic (Fluorescence PCR)
Ubumenyi bw'indwara
Kanseri y'inkondo y'umura ni kimwe mu bibyimba bibi bikunze kugaragara mu myanya myibarukiro y'abagore. Byagaragaye ko kwandura HPV idakira n'ubwandu bwinshi ari imwe mu mpamvu zikomeye zitera kanseri y'inkondo y'umura. Kuri ubu haracyari ikibazo cy’uburyo bwo kuvura kanseri y'inkondo y'umura buzwi muri rusange. Kubwibyo, kumenya no gukumira ubwandu bw'inkondo y'umura buterwa na HPV hakiri kare ni byo bintu by'ingenzi mu gukumira kanseri y'inkondo y'umura. Gushyiraho ibizamini byoroshye, byihariye kandi byihuse byo gusuzuma indwara ziterwa n'udukoko ni ingenzi cyane mu gusuzuma kanseri y'inkondo y'umura.
Umuyoboro
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Igenzura ry'imbere |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | -18℃ |
| Igihe cyo kumara | Amezi 12 |
| Ubwoko bw'ingero | Urugero rw'inkari, urugero rw'igitambaro cy'inkondo y'umura cy'umugore, urugero rw'igitambaro cy'inkari cy'umugore |
| Ct | ≤28 |
| LoD | Kopi 300/mL |
| Ubwihariye | Nta ndwara ya Ureaplasma urealyticum, Chlamydia trachomatis yo mu myanya ndangagitsina, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold, Gardnerella n'izindi ndwara za HPV zitari muri ako gakoresho. |
| Ibikoresho Bikoreshwa | Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu za PCR za SLAN-96P mu gihe nyacyo LightCycler®Sisitemu 480 za PCR mu gihe nyacyo Sisitemu zo Gusuzuma za LineGene 9600 Plus mu gihe nyacyo MA-6000 Umuvuduko w'ubushyuhe mu gihe nyacyo Sisitemu ya BioRad CFX96 ya PCR mu gihe nyacyo Sisitemu ya BioRad CFX Opus 96 ya PCR mu gihe nyacyo |
Urugendo rw'akazi
Reagent isabwa gukurwamo: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ongeraho 200μL y'umunyu usanzwe kugira ngo wongere ushyiremo agace gato mu ntambwe ya 2.1, hanyuma gukurwamo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha iyi reagent isabwa gukurwamo. Ingano ya elution isabwa ni 80μL.
Reagent yo gukuramo isabwa: QIAamp DNA Mini Kit (51304) cyangwa Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Ongeraho 200μL y'umunyu usanzwe kugira ngo wongere ushyiremo agace gato mu ntambwe ya 2.1, hanyuma gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha iyi reagent yo gukuramo. Ingano y'icyitegererezo cyakuwemo ni 200μL, kandi ingano y'icyitegererezo cyatanzwe ni 100μL.
Reagent isabwa gukurwamo: Reagent yo gusohora ingero za Macro na Micro-Test (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). Ongeraho 200μL y'ireagent yo gusohora ingero kugira ngo wongere ushyireho pellet mu ntambwe ya 2.1, hanyuma gukuramo bigomba gukorwa hakurikijwe amabwiriza yo gukoresha iyi reagent yo gusohora.
