Ubwoko 17 bwa HPV (16/8/6/11/44 kwandika)
Izina ry'ibicuruzwa
HWTS-CC015 Ubwoko bwa Papillomavirus yumuntu (16/18/6/11/44 Kwandika Acide) Acide Gutahura Acide
Epidemiology
Kanseri y'inkondo y'umura nimwe mu bibyimba bikunze kugaragara mu nzira y'imyororokere y'umugore. Byarewe ko kwandura kwandura HPV kandi indwara nyinshi ni imwe mu mpamvu zikomeye zitera kanseri y'inkondo y'umura. Kugeza ubu haracyariho kuvura muri rusange imiti ifatika ya kanseri y'inkondo y'umura yatewe na HPV. Kubwibyo, gutahura hakiri kare no kwirinda kwandura inkondor biterwa na HPV ni urufunguzo rwo kwirinda guhagarika inkoni. Gushiraho ibizamini byoroshye, byihariye kandi byihuse kubizamini byubusahuzi bifite akamaro gakomeye kubisuzumye kanseri y'inkondo y'umura.
Umuyoboro
| PCR-Mix1 | Umuryango | 18 |
| Vic / Hex | 16 | |
| Rox | 31,33,35,39,45,51,52,52,56,58.59,66,68 | |
| Cy5 | Kugenzura imbere |
| PCR-Mix2 | Umuryango | 6 |
| Vic / Hex | 11 | |
| Rox | 44 | |
| Cy5 | Kugenzura imbere |
Tekinike
| Ububiko | -18 ℃ |
| Imibereho-Ubuzima | Amezi 12 |
| Ubwoko bw'ikigereranyo | Inkari, Umugore w'inkondo y'umura w'icyitegererezo, igitsina gore cya swab icyitegererezo |
| Ct | ≤28 |
| Lod | 300copies / ml |
| Umwihariko | Nta musaraba uhurira na Ureaplasma ureelicut, Chlamydia trachomatis yo mu gice cy'imyororokere, Reisseria Goriconas, muri Trichomonas. |
| Ibikoresho bisabwa | Gushyira mu bikorwa biosystems 7500 igihe cya sisitemu ya PCR Gushyira mu bikorwa biosystems 7500 byihuse-sisitemu ya PCR Umutobe®5-igihe nyacyo PCR sisitemu Scan-96p sisitemu nyayo pcr sisitemu Umucyo®480 nyamara igihe cya PCR Linegene 9600 wongeyeho sisitemu nyayo ya PCR Ma-6000 nyayo-nyayo Biorad CFX96 Igihe cya PCR Biorad CFX OPUS 96 Igihe cya PCR |
Akazi
Gusaba Gukuramo Reagent: Macro-Ikizamini cya Viral ADN / RNA Kit (Hnats-3017-50, hwts-3017-48, HWTS-3017-96) (Birashobora gukoreshwa hamwe na macro & micro-ikizamini Automatic Autocctor ya acide (HWTS-3006C, HWTS-3006B)) na Jiagsu Macro & Micro-Ikizamini Med-Tech Co., Ltd. Ongeraho 200μl yumunyu wa saline mu buryo busanzwe bwo kwigumya pellet mu ntambwe mu ntambwe y'intambwe ya 2.1, hanyuma igura ikwiye gukorwa hakurikijwe amabwiriza yo gukoresha iyi reagen. Umubumbe w'ikirenga usabwa ni 80μl.
Gusaba gukuramo Reagent: Qiaimp DNna Mini Kit (51304) cyangwa Macro & micro-ikizamini cya virumbe ya virumbo ya virusi ya virusi ya virusi ya viruman (hwts (hwts-3020-50). Ongeramo 200μl yumunyu usanzwe wo kwigumya pellet muntambwe ya 2.1, hanyuma urwanywa zigomba gukorwa hakurikijwe amabwiriza yo gukoresha iki gikanda. Ingano yicyitegererezo yakuweho yingengo za 200μl, hamwe nubunini bwabigenewe ni 100μl.
Gusaba gukuramo Reagent: Macro & Micro-Ikizamini Icyitegererezo cya Regent (HWTS-3005-8I, HWTS-3005-8k, HWTS-3005-8L). Ongeramo 200μl of Samle Kurekura Reagent kugirango usukure pellet muntambwe ya 2.1, hanyuma urwanywa zigomba gukorwa hakurikijwe amabwiriza yo gukoresha ibi byo gukoresha.
