Ubwoko 14 bw'udukoko dutera indwara z'ubuhumekero bivanze
Izina ry'igicuruzwa
HWTS-RT159B Ubwoko 14 bw'udukoko duhumeka. Imashini ipima aside nucleic (Fluorescence PCR)
Ubumenyi bw'indwara
Indwara zo mu myanya y'ubuhumekero ni zo zikunze kugaragara cyane mu bantu, zishobora kugaragara mu gitsina icyo ari cyo cyose, mu myaka n'akarere kose. Ni imwe mu mpamvu zikomeye zitera indwara n'impfu mu baturage ku isi yose.[1]. Indwara zikunze kugaragara mu myanya y'ubuhumekero zirimo coronavirus nshya, virusi ya Grippe A, virusi ya Grippe B, virusi ya Respiratory syncytial virus, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus type I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, na Streptococcus pneumoniae, nibindi.[2,3].
Umuyoboro
| Aho iriba riherereye | Izina ry'Umuti w'Ikibazo | Udukoko twanduza indwara tugomba kumenyekana |
| 1 | Umwidagaduro w'umuhanga A | SARS-CoV-2, IFV A, IFV B |
| 2 | Master Mix B | Umujyanama, hMPV, MP, Cpn |
| 3 | Master Mix C | PIVI / II / III / IV, Rhv, RSV, HBoV |
| 4 | Master Mix D | CoV, EV, SP, igenzura ry'imbere |
| 5 | Umwidagaduro w'umuhanga A | SARS-CoV-2, IFV A, IFV B |
| 6 | Master Mix B | Umujyanama, hMPV, MP, Cpn |
| 7 | Master Mix C | PIVI / II / III / IV, Rhv, RSV, HBoV |
| 8 | Master Mix D | CoV, EV, SP, igenzura ry'imbere |
Ibipimo bya tekiniki
| Ububiko | ≤-18℃ |
| Igihe cyo kumara | Amezi 9 |
| Ubwoko bw'ingero | Igitambaro cyo mu nda, igitambaro cyo mu nda |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 2Kopi 00/mL |
| Ubwihariye | Ibisubizo by'ibizamini bya "cross-reactivity" byagaragaje ko nta "cross-reaction" yagaragaye hagati y'iyi kit na Cytomegalovirus, virusi ya Herpes simplex type 1, virusi ya Varicella-zoster, virusi ya Epstein-Barr, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, ubwoko bwa Mycobacterium tuberculosis bwagabanutse, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii na aside nucleic nucleic y'umuntu. |
| Ibikoresho Bikoreshwa | Sisitemu za PCR za SLAN-96P mu gihe nyacyo (Hongshi Medical Technology Co., Ltd.)Sisitemu za PCR zikoreshwa mu gihe nyacyo 7500 Sisitemu za PCR zikoreshwa mu buryo bwihuse 7500 QuantStudio®Sisitemu 5 za PCR mu gihe nyacyo Sisitemu zo Gupima za LineGene 9600 Plus mu gihe nyacyo (FQD-96A, ikoranabuhanga rya Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermocycler (Suzhou Molarray Co., Ltd.) Sisitemu ya BioRad CFX96 ya PCR y'igihe nyacyo, Sisitemu ya BioRad CFX Opus 96 ya PCR y'igihe nyacyo |
Urugendo rw'akazi
Reagent yo gukuramo isabwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (ishobora gukoreshwa hamwe na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ingano y'icyitegererezo cyakuwemo ni 200µL. Intambwe zikurikiraho zigomba gukorwa hakurikijwe amabwiriza yo gukoresha iyi reagent yo gukuramo. Ingano y'icyitegererezo isabwa ni80µL.
